We analyzed the mRNA expression of the insulin-like growth factor (IGF) family genes and of selected downstream pathway genes using the Affymetrix microarray system and confirmatory RT-PCR in the freshly prepared organ of Corti (OC), modiolus (MOD) and stria vascularis (SV) from neonatal rats (3-5 days old) and after 24h in culture. Among the seven members of the IGF family analyzed in this paper, IGF1, IGF2 and IGF-binding protein (IGFBP2) had the highest basal expression in all regions. Preparatory stress and culture increased the expression of IGF2, IGFBP2, IGFBP3, IGFBP5, glucose transporterl (GLUT1), signal transducer, and activator of transcription3 (STAT3), phosphoinositide-3-kinase regulatory subunit (Pik3r1), Jun oncogene (c-jun) and decreased that of mitogen-activated protein kinases MAPK3 and MAPK14 in all regions. Region-specific changes were observed in OC (GLUT1), MOD (IGFBP3 and c-jun) and SV (IGF2 and IGFBP2).

中文翻译：

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IGF系统成员在新生大鼠的Corti，modiolus和血管纹中的MRNA表达。

我们使用Affymetrix微阵列系统和经验证的RT-PCR在新鲜制备的Corti（OC），modiolus（MOD）和纹状体器官中分析了胰岛素样生长因子（IGF）家族基因和选定的下游途径基因的mRNA表达。新生大鼠（3-5天大）以及培养24小时后的血管新生（SV）。在本文分析的IGF家族的七个成员中，IGF1，IGF2和IGF结合蛋白（IGFBP2）在所有区域中具有最高的基础表达。准备压力和培养增加了IGF2，IGFBP2，IGFBP3，IGFBP5，葡萄糖转运蛋白（GLUT1），信号转导子和转录激活因子（STAT3），磷酸肌醇-3-激酶调节亚基（Pik3r1），Jun癌基因（c-jun）的表达），并在所有区域均降低了促分裂原活化蛋白激酶MAPK3和MAPK14的水平。