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Improved Method for Determining Antibiotic Susceptibility ofFlavobacterium columnareIsolates by Broth Microdilution
Journal of Aquatic Animal Health ( IF 1.2 ) Pub Date : 2008-12-01 , DOI: 10.1577/h07-047.1
Ahmed M Darwish 1 , Bradley D Farmer , John P Hawke
Affiliation  

A simple and reproducible microdilution method was developed to test the susceptibility of the bacterium Flavobacterium columnare to antibiotics in vitro. The testing was conducted at 28 degrees C for 44-48 h at two dilutions of Mueller-Hinton broth (DMHB) using a standardized inoculum, a reference isolate of Escherichia coli ATCC25922 as a quality control organism, positive and negative control wells, and standardized custom-made microtiter plates. The E. coli ATCC25922 and F. columnare ATCC23463 (the species type strain) had significantly better growth in DMHB at 1:5 (4 g/L) than at 1:7 (3 g/L). The E. coli ATCC25922 was found to be acceptable as a reference isolate and produced minimum inhibitory concentration (MIC) values similar to those in the range published by the Clinical and Laboratory Standards Institute derived using standard Mueller-Hinton broth. The new method was used to determine the MIC of 23 F. columnare isolates (representing the three genotypes of the species) to enrofloxacin, ampicillin, oxytetracycline, erythromycin, florfenicol, flumequine, ormetoprim/sulfadimethoxine, and oxolinic acid.

中文翻译:

肉汤微量稀释法测定柱状黄杆菌菌株抗生素敏感性的改进方法

开发了一种简单且可重复的微量稀释方法来测试柱状黄杆菌在体外对抗生素的敏感性。使用标准化接种物、大肠杆菌 ATCC25922 的参考分离物作为质量控制生物、阳性和阴性对照孔以及标准化的两种稀释度的 Mueller-Hinton 肉汤 (DMHB) 在 28 摄氏度下进行 44-48 小时的测试。定制的微量滴定板。E.coli ATCC25922 和 F. columnare ATCC23463(物种类型菌株)在 DMHB 1:5 (4 g/L) 中的生长明显优于 1:7 (3 g/L)。E。发现大肠杆菌 ATCC25922 作为参考分离物是可以接受的,并且产生的最低抑菌浓度 (MIC) 值与使用标准 Mueller-Hinton 肉汤得出的临床和实验室标准研究所公布的范围内的值相似。新方法用于确定 23 F. columnare 分离株(代表该物种的三种基因型)对恩诺沙星、氨苄青霉素、土霉素、红霉素、氟苯尼考、氟甲喹、奥美托普林/磺胺二甲氧嘧啶和恶唑酸的 MIC。
更新日期:2008-12-01
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