当前位置: X-MOL 学术Electromagn. Biol. Med. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Variability of the Minimal Transmembrane Voltage Resulting in Detectable Membrane Electroporation
Electromagnetic Biology and Medicine ( IF 1.7 ) Pub Date : 2008-01-01 , DOI: 10.1080/15368370802394644
Leila Towhidi 1 , Tadej Kotnik , Gorazd Pucihar , S M P Firoozabadi , Hossein Mozdarani , Damijan Miklavcic
Affiliation  

We present a study of the variability of the minimal transmembrane voltage resulting in detectable electroporation of the plasma membrane of spherical and irregularly shaped CHO cells (we denote this voltage by ITVc). Electroporation was detected by monitoring the influx of Ca2+, and the transmembrane voltage was computed on a 3D finite-elements model of each cell constructed from its cross-section images. We found that ITVc was highly variable, particularly in irregularly shaped cells, where it ranged from 512–1028 mV. We show that this range is much too large to be an artifact due to numerical errors and experimental inaccuracies, implying that for cells of the same type and exposed to the same number of pulses with the same duration, the value of ITVc can differ considerably from one cell to another. We also observed that larger cells are in many cases characterized by a higher ITVc than a smaller one. This is in qualitative agreement with the reports that higher membrane curvature facilitates electroporation, but quantitative considerations suggest that the observed variability of ITVc cannot be attributed entirely to the differences in membrane curvature.

中文翻译:

最小跨膜电压的可变性导致可检测的膜电穿孔

我们研究了最小跨膜电压的可变性,导致球形和不规则形状 CHO 细胞质膜的可检测电穿孔(我们用 ITVc 表示该电压)。通过监测 Ca2+ 的流入来检测电穿孔,并在每个细胞的 3D 有限元模型上计算跨膜电压,该模型由其横截面图像构建。我们发现 ITVc 变化很大,特别是在形状不规则的细胞中,其范围为 512-1028 mV。我们表明,由于数值误差和实验不准确,这个范围太大而不能成为伪影,这意味着对于相同类型的细胞并暴露于相同持续时间的相同脉冲数,ITVc 的值可能与一个单元格到另一个单元格。我们还观察到,在许多情况下,较大的细胞比较小的细胞具有更高的 ITVc。这与更高的膜曲率促进电穿孔的报道定性一致,但定量考虑表明观察到的 ITVc 变异性不能完全归因于膜曲率的差异。
更新日期:2008-01-01
down
wechat
bug