The production of diffraction quality crystals for the structural determination of inner membrane proteins relies on obtaining large amounts of stable protein. Achieving this, by finding the correct parameters to successfully express and purify these proteins is often time-consuming and frustrating. The methods described here examine the most important parameters, in both expression and purification, quickly and simply. They take into account methods previously used in successful structural determinations of inner membrane proteins and collect and analyse data for use in further experiments and to investigate overall trends. These methods make use of histidine-tagged membrane proteins with a green fluorescent protein fusion but could be adapted easily for other proteins.

中文翻译：

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用于表达和纯化内膜蛋白的高通量方法的协议。

用于确定内膜蛋白结构的衍射质量晶体的生产依赖于获得大量稳定的蛋白。通过找到正确的参数以成功表达和纯化这些蛋白质来实现这一目标通常是耗时且令人沮丧的。此处描述的方法可以快速，简单地检查表达和纯化中最重要的参数。他们考虑到了以前成功用于内膜蛋白结构测定的方法，并收集和分析了用于进一步实验和研究总体趋势的数据。这些方法将带有组氨酸标签的膜蛋白与绿色荧光蛋白融合物一起使用，但可以轻松地用于其他蛋白。