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Signal transduction pathways and haem oxygenase induction in soybean leaves subjected to salt stress.
Redox Report ( IF 3.8 ) Pub Date : 2008-11-20 , DOI: 10.1179/135100008x308966
K B Balestrasse 1 , C G Zilli , M L Tomaro
Affiliation  

We have previously demonstrated that the induction of haem oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against cadmium and UV-B stress. Here, we have investigated the possible signal transduction pathways involved in haem oxygenase-1 induction in leaves of soybean plants subjected to salt stress. Treatment with 100 mM NaCl during 48 h increased thiobarbituric acid reactive substances by 30%, whereas GSH decreased by 50%, with respect to controls. These effects were prevented by pre-incubation with diphenyleneiodonium (DPI; an NADPH oxidase inhibitor), [1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ; a guanylate cyclase inhibitor) or LaCl3 (calcium channel blocker). NaCl at 100 mM produced in situ accumulation of H2O2 and O2*-, which were also prevented by DPI, ODQ or LaCl3. Moreover, salt-induced haem oxygenase-1 activity was also totally abolished by pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress triggered by salt stress were similar to those implicated in haem oxygenase-1 induction, and provide additional information suggesting that haem oxygenase might play a key role in the antioxidative protection machinery of higher plants.

中文翻译:

盐胁迫下大豆叶片的信号转导途径和血红素加氧酶的诱导。

先前我们已经证明,血红素加氧酶-1(EC 1.14.99.3)的诱导对大豆植物具有抗镉和UV-B胁迫的保护作用。在这里,我们研究了盐胁迫下大豆叶片中血红素加氧酶-1诱导中可能的信号转导途径。与对照组相比,在48小时内用100 mM NaCl处理可使硫代巴比妥酸反应性物质增加30%,而GSH减少50%。通过与二亚苯基碘鎓(DPI; NADPH氧化酶抑制剂),[1,2,4]恶二唑-[4,3-a]喹喔啉-1-酮(ODQ;鸟苷酸环化酶抑制剂)或LaCl3预先孵育可防止这些作用(钙通道阻滞剂)。100 mM的NaCl原位产生H2O2和O2 *-积累,这也可以通过DPI,ODQ或LaCl3阻止。此外,用不同的抑制剂预处理也完全消除了盐诱导的血红素加氧酶-1活性。这些结果清楚地表明,盐胁迫触发的氧化应激涉及的信号转导途径与血红素加氧酶-1诱导中涉及的信号转导途径相似,并提供了更多信息,表明血红素加氧酶可能在高等植物的抗氧化保护机制中起关键作用。 。
更新日期:2019-11-01
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