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Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis.
Molecular Membrane Biology ( IF 2.857 ) Pub Date : 2008-11-05 , DOI: 10.1080/09687680802448530
R M Cleverley 1 , M Saleem , J Kean , R C Ford , J P Derrick , S M Prince
Affiliation  

A method to rapidly assess the oligomeric composition of multimeric proteins is notably absent from reported schemes for high throughput production and crystallization of membrane proteins. In this report we have investigated the suitability of PFO-PAGE electrophoresis for this purpose and present examples where it proves highly informative in selecting conditions favouring the functional oligomeric state of the target protein. Features such as the ability to analyze several samples in parallel, including crude membrane extracts, suggest it will be highly adaptable to high throughput analysis of membrane proteins.

中文翻译:

使用PFO-PAGE电泳选择要结晶的膜蛋白靶标。

快速报道的多聚体蛋白质的寡聚体组成的评估方法明显缺乏用于高通量生产和膜蛋白结晶的报道方案。在本报告中,我们研究了PFO-PAGE电泳是否适合该目的,并提供了一些实例,在选择有利于目标蛋白功能性低聚状态的条件下,它具有很高的参考价值。诸如并行分析多个样品(包括粗膜提取物)的能力等功能表明,它将高度适用于膜蛋白的高通量分析。
更新日期:2019-11-01
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