During 2004 and 2005 a survey was conducted to investigate the presence and geographic distribution of largemouth bass virus (LMBV) in New York State. This iridovirus is widely distributed across the eastern United States; however, it had not previously been reported in New York State. Two hundred and eighty-three wild largemouth bass Micropterus salmoides and 8 smallmouth bass M. dolomieu were collected from 37 locations across the state. No clinical signs of LMBV or mortalities attributable to the virus were observed in the fish collected. Using a quantitative polymerase chain reaction (QPCR) method, we detected LMBV in 28 fish from 13 locations. Viral cytopathic effect in cell culture was observed in 5 fish from 3 locations. The virus isolated from cell culture was confirmed to be LMBV by an independent PCR method. Statistical analysis of the largemouth bass samples collected during 2005 revealed a wide difference in prevalence between the QPCR results and the cell culture results. Analysis of possible predictors, including age, sex, and month collected, showed no significant associations with the QPCR results. This survey confirms the presence and wide distribution of a potentially pathogenic form of LMBV in multiple water systems across New York State.

中文翻译：

---

确定纽约州野生淡水鲈鱼中大口黑鲈病毒存在和分布的调查

在 2004 年和 2005 年期间，进行了一项调查，以调查纽约州大口黑鲈病毒 (LMBV) 的存在和地理分布。这种虹彩病毒广泛分布于美国东部；然而，此前在纽约州没有报道过。从全州 37 个地点收集了 283 条野生大嘴黑鲈 Micropterus salmoides 和 8 条小嘴黑鲈 M. dolomieu。在收集的鱼中没有观察到 LMBV 的临床症状或病毒导致的死亡。使用定量聚合酶链反应 (QPCR) 方法，我们在来自 13 个地点的 28 条鱼中检测到 LMBV。在来自 3 个地点的 5 条鱼中观察到细胞培养物中的病毒致细胞病变效应。从细胞培养物中分离的病毒通过独立的 PCR 方法被确认为 LMBV。2005 年收集的大口黑鲈样本的统计分析显示，QPCR 结果和细胞培养结果之间的流行率存在很大差异。对可能的预测因素（包括收集的年龄、性别和月份）的分析显示与 QPCR 结果没有显着关联。该调查证实了纽约州多个水系统中存在潜在致病性 LMBV 形式并广泛分布。