Seminal sperm-agglutinating antibodies along with IgG antibodies against laminin-1 and intraacrosomal sperm proteins were examined in seventy-one men from infertile couples. The direct mixed antiimmunoglobulin reaction test for IgG, IgA, and the commercial ELISA method for detecting IgG antibodies against laminin-1 in seminal plasma were used. Intraacrosomal proteins in the sperm heads were detected by immunofluorescence using monoclonal antibodies. Cellular elements other than spermatozoa, collectively referred to as "round cells" were also examined. In association with a group of oligoasthenospermatic men, positive levels (44%) of antibodies against laminin-1 of the IgG isotype in seminal plasma were found in conjunction with increased cellularity in semen. Interestingly, the elevated levels of anti-laminin-l IgG and sperm agglutinating positivity were not correlated. The use of antibodies against sperm antigens targeted to adhesive molecules such as laminin-1 contributes to diagnosing reproductive failure. Detection of intraacrosomal proteins very often correlates to the state of semen pathology and inflammation.

中文翻译：

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针对不育夫妇精液中层粘连蛋白1和精子，精子内蛋白的抗体。

在71名不育夫妇的男性中检查了精子凝集抗体以及针对层粘连蛋白-1和精子内精子蛋白的IgG抗体。使用了IgG，IgA的直接混合抗免疫球蛋白反应测试，以及用于检测精浆中层粘连蛋白-1 IgG抗体的商业化ELISA方法。使用单克隆抗体通过免疫荧光检测精子头部的顶体内蛋白。还检查了除精子以外的其他细胞成分，统称为“圆形细胞”。与一组少精子症男性患者相关，在精浆中发现阳性水平（44％）的抗IgG Laminin-1 IgG同种型抗体与精液细胞增多有关。有趣的是 抗层粘连蛋白-1 IgG的水平升高与精子凝集阳性无关。针对针对粘附分子（如层粘连蛋白-1）的精子抗原的抗体的使用有助于诊断生殖衰竭。顶体蛋白的检测通常与精液病理和炎症状态相关。