Swimming in reduced electrophysiological preparations of the pteropod mollusc, Clione limacina, was blocked by bath application of hexamethonium even though pattern generator activity continued with this treatment. Neuromuscular recordings indicated that hexamethonium blocked synaptic input from Pd-3 and Pd-4 motoneurons to slow-twitch muscle cells, while connections from Pd-1A and Pd-2A motoneurons to fast-twitch muscle cells were variable in their response to hexamethonium-synaptic inputs were suppressed in most cases and occasionally blocked, but the latter only with high concentrations and long incubations. Acutely dissociated wing muscle cells showed a concentration-dependency in the percentage of contracted cells with bath application of acetylcholine, and this contractile activity was blocked in preparations that were first bathed in hexamethonium. Intracellular recordings from dissociated slow-twitch muscle cells showed conductance-increase depolarizations of approximately 20 mV following 1 s pressure ejections of 10(-4) M acetylcholine from micropipettes placed immediately adjacent to the muscle cells. These responses were blocked when hexamethonium was bath applied prior to the pressure-applied acetylcholine. The results suggest the Pd-3/Pd-4 motoneuron to slow-twitch muscle cell junctions are cholinergic with nicotinic-like receptors, while the Pd-1A/Pd-2A to fast-twitch muscle cell connections are likely cholinergic, but with a different receptor type.

中文翻译：

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翼足类软体动物Clione limacina的游泳肌肉组织对六甲铵的敏感性。

即使在这种处理下模式产生器活动仍在继续，六足类软体动物，Clione limacina的减少的电生理制剂中的游泳也被浴液阻止了。神经肌肉记录表明，六甲铵阻止了Pd-3和Pd-4运动神经元向慢肌神经细胞的突触输入，而Pd-1A和Pd-2A运动神经元与快肌神经细胞的连接在其对六甲铵突触的反应中是可变的。在大多数情况下，输入被抑制，偶尔会被阻止，但后者只有在高浓度和长时间孵育后才被阻止。急性离解的翼肌细胞在浴液中加入乙酰胆碱后，收缩细胞的百分比呈浓度依赖性，最初浸泡在六甲铵中的制剂阻止了这种收缩活性。离解的慢肌肌肉细胞的细胞内记录显示电导增加去极化约20毫伏后1秒从紧邻肌肉细胞放置的微量移液器中喷出10（-4）M乙酰胆碱。当在加压施加乙酰胆碱之前先浸入六甲铵时，这些反应被阻断。结果表明，Pd-3 / Pd-4运动神经元与慢肌肌肉连接处具有烟碱样受体的胆碱能，而Pd-1A / Pd-2A与快肌细胞连接可能与胆碱能有关，但具有神经毒性。不同的受体类型。离解的慢肌肌肉细胞的细胞内记录显示电导增加去极化约20毫伏后1秒从紧邻肌肉细胞放置的微量移液器中喷出10（-4）M乙酰胆碱。当在加压施加乙酰胆碱之前先浸入六甲铵时，这些反应被阻断。结果表明，Pd-3 / Pd-4运动神经元与慢肌肌肉连接处具有烟碱样受体的胆碱能，而Pd-1A / Pd-2A与快肌细胞连接可能与胆碱能有关，但具有神经毒性。不同的受体类型。离解的慢肌肌肉细胞的细胞内记录显示电导增加去极化约20毫伏后1秒从紧邻肌肉细胞放置的微量移液器中喷出10（-4）M乙酰胆碱。当在加压施加乙酰胆碱之前先浸入六甲铵时，这些反应被阻断。结果表明，Pd-3 / Pd-4运动神经元与慢肌肌肉连接处具有烟碱样受体的胆碱能，而Pd-1A / Pd-2A与快肌细胞连接可能与胆碱能有关，但具有神经毒性。不同的受体类型。