The aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (REP-PCR) and repetitive intergenic consensus PCR (ERIC-PCR), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, Pseudomonas anguilliseptica and Aeromonas salmonicida. A total of 30 A. salmonicida and 52 P. anguilliseptica were analyzed. For P. anguilliseptica, three different major fingerprints were obtained with both techniques, which defined three genomic groups: one was composed of strains isolated from eels Anguilla spp., the second of strains from turbot Scophthalmus maximus and blackspot seabream (also known as red seabream) Pagellus bogaraveo, and the third of strains from other fish species, such as gilthead seabream (also known as gilthead bream) Sparus auratus, sea bass Dicentrarchus labrax (also known as European bass Morone labrax), and salmonids. In the case ofA. salmonicida, promising results were obtained with both techniques for subspecies differentiation. Thus, two genomic profiles were obtained by ERIC-PCR. The first profile consisted of A. salmonicida subsp. salmonicida strains isolated from the different hosts. The second profile was composed of two A. salmonicida subsp. masoucida and one A. salmonicida subsp. achromogenes. Using REP-PCR, three genotypes were obtained within this pathogen that were related to the diverse subspecies analyzed. In summary, both methodologies are useful for typing distinct strains associated with different host species and therefore are helpful in epidemiological studies of P. anguilliseptica. In contrast, in the case of A. salmonicida, more studies are needed to determine their utility in discriminating the subspecies salmonicida from the other two subspecies.

中文翻译：

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聚合酶链反应扩增重复基因间共识和重复基因外回文序列，用于对假单胞菌和鲑鱼气单胞菌进行分子分型

本研究的目的是评估两种分子技术的使用，即重复基因外回文聚合酶链反应 (REP-PCR) 和重复基因间共有 PCR (ERIC-PCR)，作为流行病学工具来区分两个内的遗传不同菌株。细菌性鱼类病原体、假单胞菌和杀鲑气单胞菌。总共分析了 30 种杀鲑鱼和 52 种鳗鲡。对于 P. anguilliseptica，两种技术都获得了三种不同的主要指纹，这些指纹定义了三个基因组：一种由从鳗鱼 Anguilla spp. 中分离的菌株组成，另一种由来自大菱鲆和黑斑鲷（也称为红鲷）的菌株组成) Pagellus bogaraveo，以及来自其他鱼类品种的第三种菌株，如金头鲷（又名gilthead bream）Sparus auratus、鲈鱼Dicentrarchus labrax（又名欧洲鲈鱼Morone labrax）、鲑鱼等。在A的情况下。杀鲑鱼，两种亚种分化技术都获得了有希望的结果。因此，通过 ERIC-PCR 获得了两个基因组图谱。第一个配置文件由杀鲑鱼亚种组成。从不同宿主中分离的杀鲑鱼菌株。第二个配置文件由两个 A. salicida subsp. 组成。masoucida 和一种 A. 鲑鱼亚种。无色基因。使用 REP-PCR，在该病原体内获得了与所分析的不同亚种相关的三种基因型。总之，这两种方法都可用于对与不同宿主物种相关的不同菌株进行分型，因此有助于 P. anguilliseptica 的流行病学研究。