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Protein secretion and outer membrane assembly in Alphaproteobacteria.
FEMS Microbiology Reviews ( IF 11.3 ) Pub Date : 2008-08-28 , DOI: 10.1111/j.1574-6976.2008.00130.x
Xenia Gatsos 1 , Andrew J Perry , Khatira Anwari , Pavel Dolezal , P Peter Wolynec , Vladimir A Likić , Anthony W Purcell , Susan K Buchanan , Trevor Lithgow
Affiliation  

The assembly of beta-barrel proteins into membranes is a fundamental process that is essential in Gram-negative bacteria, mitochondria and plastids. Our understanding of the mechanism of beta-barrel assembly is progressing from studies carried out in Escherichia coli and Neisseria meningitidis. Comparative sequence analysis suggests that while many components mediating beta-barrel protein assembly are conserved in all groups of bacteria with outer membranes, some components are notably absent. The Alphaproteobacteria in particular seem prone to gene loss and show the presence or absence of specific components mediating the assembly of beta-barrels: some components of the pathway appear to be missing from whole groups of bacteria (e.g. Skp, YfgL and NlpB), other proteins are conserved but are missing characteristic domains (e.g. SurA). This comparative analysis is also revealing important structural signatures that are vague unless multiple members from a protein family are considered as a group (e.g. tetratricopeptide repeat (TPR) motifs in YfiO, beta-propeller signatures in YfgL). Given that the process of the beta-barrel assembly is conserved, analysis of outer membrane biogenesis in Alphaproteobacteria, the bacterial group that gave rise to mitochondria, also promises insight into the assembly of beta-barrel proteins in eukaryotes.

中文翻译:

Alphaproteobacteria 中的蛋白质分泌和外膜组装。

β-桶蛋白组装成膜是革兰氏阴性细菌、线粒体和质体必不可少的基本过程。我们对 β 桶组装机制的理解正在从在大肠杆菌和脑膜炎奈瑟菌中进行的研究中取得进展。比较序列分析表明,虽然介导 β-桶蛋白组装的许多成分在所有具有外膜的细菌群中都是保守的,但某些成分明显不存在。特别是 Alphaproteobacteria 似乎易于基因丢失,并显示出介导 β-桶组装的特定成分的存在或不存在:整个细菌群(例如 Skp、YfgL 和 NlpB)中似乎缺少该途径的某些成分,其他蛋白质是保守的,但缺少特征域(例如 SurA)。这种比较分析还揭示了重要的结构特征,除非将蛋白质家族的多个成员视为一个组,否则这些特征是模糊的(例如,YfiO 中的四肽重复 (TPR) 基序,YfgL 中的β-螺旋桨特征)。鉴于 β 桶组装过程是保守的,对 Alphaproteobacteria(产生线粒体的细菌群)中外膜生物发生的分析也有望深入了解真核生物中 β 桶蛋白的组装。
更新日期:2019-11-01
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