Fertilized eggs collected from broodfish infected by Ovipleistophora ovariae were tested by quantitative polymerase chain reaction (PCR) and found to be positive for the O. ovariae genome at 7.77 X 10(2) to 3.26 x 10(7) copies per microgram of host DNA. Fry hatched from these eggs contained from 1.37 X 10(2) to 9.89 X 10(6) copies of the O. ovariae genome per microgram of host DNA. Surface treatments of fertilized eggs with 150 mg formalin/L (used by farms as a fungicide) or a 1.5% solution of sodium sulfite (which removes the adhesive egg matrix) did not reduce vertical transmission to fry. Treatment of eggs with a 10% solution of bleach or a proprietary commercial DNA denaturant did not reduce the number of egg-associated copies of the O. ovariae genome. Histology of ovaries of infected fish demonstrated spores within the oocytes. However, no spores were observed by histology in positive fry hatched from infected eggs. The PCR and histological demonstration of the presence of O. ovariae spores in oocytes and fry, and the failure of strong DNA denaturants to reduce egg-associated copies, give evidence that O. ovariae is vertically transmitted within eggs.

中文翻译：

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Ovipleistophora ovariae（小孢子虫）在金光子卵内的垂直传播

通过定量聚合酶链反应 (PCR) 测试从感染卵子虫感染的亲鱼中收集的受精卵，发现 O. ovariae 基因组呈阳性，每微克宿主 DNA 拷贝数为 7.77 X 10(2) 至 3.26 x 10(7) . 从这些卵孵化的鱼苗每微克宿主 DNA 包含 1.37 X 10(2) 到 9.89 X 10(6) 个 O. ovariae 基因组拷贝。用 150 毫克福尔马林/升（农场用作杀菌剂）或 1.5% 的亚硫酸钠溶液（去除粘附的蛋基质）对受精卵进行表面处理并没有减少垂直传播到煎炸。用 10% 的漂白剂溶液或专有的商业 DNA 变性剂处理鸡蛋并没有减少 O. ovariae 基因组的鸡蛋相关拷贝数。受感染鱼卵巢的组织学显示卵母细胞内有孢子。然而，在从受感染的卵孵化的阳性鱼苗中，组织学未观察到孢子。卵母细胞和鱼苗中存在 O. ovariae 孢子的 PCR 和组织学证明，以及强 DNA 变性剂未能减少卵相关拷贝，证明 O. ovariae 在卵内垂直传播。