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A study of the interaction of oligonucleotides with polynucleotides by the method of equilibrium gel filtration. The effect of magnesium ions and the composition of the oligonucleotides on the stability of the complex.
Molecular Biology ( IF 1.2 ) Pub Date : 1975-01-01
V M Chernaenko , S E Bresler

The interaction of the oligonucleotides ApApA, ApApU and ApApC with polyribouridylic acid and of hexariboinosinic acid with and without phosphate near the 3'-end with polyribocytidylic acid was studied by the method of equilibrium gel filtration through Sephadexes. The free energy, the energy and entropy of the complexing were calculated from isotherms of the adsorption of the oligonucleotides on the polynucleotides in relation to the composition of the oligonucleotides and the concentration of magnesium ions in the buffer. It was shown that in the case of the interaction of ApApA and ApApC with poly(U) a perfect triple complex is formed, while in the case of the interaction of ApApU with poly(U) the noncomplementary base is partially displaced from the complex. The free energy of the interaction of neighboring oligonucleotides in a complex with polynucleotides is from --1000 to --3000 cal/mole depending on the type of the complex. It was shown that after the interaction of hexainosinic acid with poly(C) a double complex is formed. The free energy of the interaction of neighboring oligonucleotides in this complex is formed. The free energy of the interaction of neighboring oligonucleotides in this complex is about --1200 cal/mole. Magnesium ions have a different effect on the formation of triple and double complexes in the interaction of oligonucleotides with polynucleotides.

中文翻译:

通过平衡凝胶过滤方法研究寡核苷酸与多核苷酸的相互作用。镁离子和寡核苷酸组成对复合物稳定性的影响。

通过通过葡聚糖凝胶进行平衡凝胶过滤的方法,研究了寡核苷酸ApApA,ApApU和ApApC与聚核糖苷酸和六核糖苷酸在3'端附近有和没有磷酸盐的情况下与聚核糖苷酸的相互作用。从寡核苷酸在多核苷酸上的吸附等温线,根据寡核苷酸的组成和缓冲液中镁离子的浓度,计算出自由能,复合物的能量和熵。结果表明,在ApApA和ApApC与poly(U)相互作用的情况下,形成了完美的三重复合物,而在ApApU与poly(U)相互作用的情况下,非互补碱基部分地从复合物中置换出来。复杂物中相邻寡核苷酸与多核苷酸相互作用的自由能为-1000到-3000 cal / mol,取决于复杂物的类型。结果表明,己糖酸与聚(C)相互作用后,形成了双络合物。形成该复合物中相邻寡核苷酸相互作用的自由能。该复合物中相邻寡核苷酸相互作用的自由能约为--1200卡/摩尔。在寡核苷酸与多核苷酸的相互作用中,镁离子对三重和双重复合物的形成具有不同的影响。形成该复合物中相邻寡核苷酸相互作用的自由能。该复合物中相邻寡核苷酸相互作用的自由能约为--1200卡/摩尔。在寡核苷酸与多核苷酸的相互作用中,镁离子对三重和双重复合物的形成具有不同的影响。形成该复合物中相邻寡核苷酸相互作用的自由能。该复合物中相邻寡核苷酸相互作用的自由能约为--1200卡/摩尔。在寡核苷酸与多核苷酸的相互作用中,镁离子对三重和双重复合物的形成具有不同的影响。
更新日期:2019-11-01
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