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A study of the mechanism of action of phosphorylase B using isotope-labelled substrates.
Molecular Biology ( IF 1.2 ) Pub Date : 1975-01-01
L M Firsov , T I Bogacheva , S E Bresler

We have measured the alpha-deuterium and alpha-tritium secondary kinetic isotope effects on the synthesis and phosphorolysis of glycogen catalyzed by glycogen phosphorylase B (EC 2.4.1.1). The isotope-labeled substrates glucose-1T-1-phosphate and 1C14-glucose-1D-1-phosphate were used in the experiments, together with 6T-glucose-1-phosphate and C14-glucose-1-phosphate as controls. Measurement by the double label technique showed that both effects studied were equal to zero within the limits of experimental error. The possible mechanism of action of phosphorylase B is discussed.

中文翻译:

使用同位素标记的底物研究磷酸化酶B的作用机理。

我们已经测量了α-氘和α-tri次生动力学同位素对糖原磷酸化酶B(EC 2.4.1.1)催化的糖原合成和磷酸分解的影响。实验中使用了同位素标记的底物葡萄糖-1T-1-磷酸和1C14-葡萄糖-1D-1-磷酸,以及6T-葡萄糖-1-磷酸和C14-葡萄糖-1-磷酸作为对照。通过双标签技术进行的测量表明,在实验误差的范围内,所研究的两种效应均等于零。讨论了磷酸化酶B作用的可能机制。
更新日期:2019-11-01
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