The ability of decomposed rat liver mitochondria, stripped of their outer membrane (fracton C) to bind 14C-DNA of phage T4 was demonstrated. The bound phage DNA is 10-15% resistant to treatment of fraction C with DNAase I. Treatment of fraction C with sarcosyl (final concentration 0.1%) and centrifugation in a linear sucrose gradient (20-50%) permits the detection of a label in the Mt-DNA-membrane complex, isolated at 35% sucrose. Further fractionation of the membrane complex on sepharose 4 B promotes the detection of the label in "elementary particles of transcription" [1], structural units of the inner membrane, containing specific centers for binding to DNA. The centers of binding of the elementary particles of transcription possess greatest affinity for Mt-DNA; however, they are also capable of binding DNA of various origin (both the DNA of eucaryotes and that of procaryotes). The significance of fixation of DNA of nonmitochondrial origin by the membrane structures of the mitochondria is discussed.

中文翻译：

---

线粒体的膜结构与非线粒体来源的DNA之间形成复合物。

证明了分解大鼠肝线粒体的能力，剥夺了它们的外膜（C部分）结合噬菌体T4的14C-DNA。结合的噬菌体DNA对用DNAase I处理级分C的抗性为10-15％。用肌氨酰（终浓度为0.1％）处理级分C，并以线性蔗糖梯度（20-50％）离心可检测标记Mt-DNA-膜复合物中的糖原分离为35％蔗糖。在琼脂糖凝胶4 B上进一步分离膜复合物，促进了对“转录的基本粒子” [1]（内膜的结构单元，包含与DNA结合的特定中心）的标记的检测。转录基本粒子的结合中心对Mt-DNA具有最大的亲和力。然而，它们还能够结合各种来源的DNA（真核生物和原核生物的DNA）。讨论了通过线粒体的膜结构固定非线粒体DNA的重要性。