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Renaturation of DNA from ultrasonically fragmented chromatin.
Molecular Biology ( IF 1.2 ) Pub Date : 1975-01-01
I P Ashmarin , N A Fedorova , E G Karpukhina

The rates of renaturation of ultrasonically treated DNA, isolated from fragments of rat liver chromatin, were compared. Chromatographic fractionation on hydroxylapatite was used to fractionate the denatured and renatured DNAs. It was shown that DNA fraction 1 renatures considerably faster than fraction 2. The results are compared with previously published data on the specificity coefficient of DNA, the content of basic amino acids and alkali-labile phosphorus of the protein in the corresponding fragments of the chromatin. The possible functional role of these fragments in the genome is discussed.

中文翻译:

从超声破碎的染色质中DNA复性。

比较了从大鼠肝染色质片段中分离的超声处理过的DNA的复性率。使用羟磷灰石的色谱分离来分离变性和复性的DNA。结果表明,DNA组分1的复性速度比组分2快得多。将结果与先前发表的有关染色质相应片段中DNA的特异性系数,碱性氨基酸含量和蛋白质的碱金属磷的数据进行了比较。 。讨论了这些片段在基因组中的可能功能。
更新日期:2019-11-01
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