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A Microfluidic Device for Continuous-Flow Magnetically Controlled Capture and Isolation of Microparticles
Journal of Microelectromechanical Systems ( IF 2.7 ) Pub Date : 2010-08-01 , DOI: 10.1109/jmems.2010.2050194
Yao Zhou 1 , Yi Wang 2 , Qiao Lin 1
Affiliation  

This paper presents a novel microfluidic device that exploits magnetic manipulation for integrated capture and isolation of microparticles in continuous flow. The device, which was fabricated from poly(dimethylsiloxane) (PDMS) by soft-lithography techniques, consists of an incubator and a separator integrated on a single chip. The incubator is based on a novel scheme termed target acquisition by repetitive traversal (TART), in which surface-functionalized magnetic beads repetitively traverse a sample to seek out and capture target particles. This is accomplished by a judicious combination of a serpentine microchannel geometry and a time-invariant magnetic field. Subsequently, in the separator, the captured target particles are isolated from nontarget particles via magnetically driven fractionation in the same magnetic field. Due to the TART incubation scheme that uses a corner-free serpentine channel, the device has no dead volume and allows minimization of undesired particle or magnetic-bead retention. Single-chip integration of the TART incubator with the magnetic-fractionation separator further allows automated continuous isolation and retrieval of specific microparticles in an integrated manner that is free of manual off-chip sample incubation, as often required by alternative approaches. Experiments are conducted to characterize the individual incubation and separation components, as well as the integrated device. The device is found to allow 90% of target particles in a sample to be captured and isolated and 99% of nontarget particles to be eliminated. With this high separation efficiency, along with excellent reliability and flexibility, the device is well suited to sorting, purification, enrichment, and detection of micro/nanoparticles and cells in lab-on-a-chip systems.

中文翻译:

用于连续流动磁控捕获和分离微粒的微流体装置

本文提出了一种新型微流体装置,该装置利用磁操纵在连续流动中对微粒进行集成捕获和分离。该设备由聚二甲基硅氧烷 (PDMS) 通过软光刻技术制成,由集成在单个芯片上的孵化器和分离器组成。该孵化器基于一种称为重复遍历目标获取 (TART) 的新方案,其中表面功能化的磁珠重复遍历样本以寻找和捕获目标粒子。这是通过蛇形微通道几何形状和时不变磁场的明智组合来实现的。随后,在分离器中,通过在同一磁场中的磁驱动分级分离捕获的目标颗粒与非目标颗粒。由于使用无角蛇形通道的 TART 孵化方案,该设备没有死体积,并可以最大限度地减少不需要的颗粒或磁珠滞留。TART 培养箱与磁分离分离器的单芯片集成进一步允许以集成方式自动连续分离和检索特定微粒,无需手动芯片外样品培养,这通常是替代方法所要求的。进行实验以表征各个孵育和分离组件以及集成设备。发现该装置可以捕获和分离样品中 90% 的目标粒子,并消除 99% 的非目标粒子。凭借这种高分离效率以及出色的可靠性和灵活性,
更新日期:2010-08-01
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