One of an essential characteristic of human skin are time dependent mechanical properties. Here, we demonstrate that stiffness of human dermal fibroblast correlates with age and it can be restored after anti-wrinkle tripeptide treatment. The stiffness of human fibroblasts isolated from donors of 30-, 40- and 60 years old were examined. Additionally the effect of anti- wrinkle tripeptide of latter cells was investigated. The atomic force microscopy measurements were performed on untreated fibroblast as well as on treated with the peptide. The Young’s modulus for two indentation depths 200 and 600 nm of each cell type was determined. The Young’s modulus increases with age of the cells. The highest values of Young’s modulus were obtained for fibroblasts collected from 60 years old donors, for indentation depth of ~200 nm. For larger indentation depth of 600 nm there are no significant differences in stiffness between cells. Fibroblasts treated with the anti-wrinkle tripeptide exhibit lower Young’s modulus. The cells derived from 40- and 60-years old donors restored stiffness characteristic to the level of 30 years old subjects. The results show correlation between stiffness and age of the human fibroblast as well as impact of anti-wrinkle tripeptide on the mechanical properties of skin cells.

中文翻译：

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抗皱三肽治疗后人类成纤维细胞机械特性的年龄相关变化及其预期逆转。

人类皮肤的基本特征之一是随时间变化的机械特性。在这里，我们证明了人皮肤成纤维细胞的硬度与年龄相关，并且可以在抗皱三肽治疗后恢复。检查了从 30 岁、40 岁和 60 岁供体中分离的人类成纤维细胞的硬度。另外研究了后面细胞的抗皱三肽的作用。对未处理的成纤维细胞以及用肽处理的成纤维细胞进行原子力显微镜测量。测定了每种细胞类型的两个压痕深度 200 和 600 nm 的杨氏模量。杨氏模量随着细胞的老化而增加。杨氏模量的最高值是从 60 岁供体收集的成纤维细胞中获得的，压痕深度约为 200 nm。对于 600 nm 的较大压痕深度，单元之间的刚度没有显着差异。用抗皱三肽处理的成纤维细胞表现出较低的杨氏模量。来自 40 岁和 60 岁供体的细胞将刚度特征恢复到 30 岁受试者的水平。结果显示了人成纤维细胞的硬度和年龄之间的相关性，以及抗皱三肽对皮肤细胞机械性能的影响。