Multifocal multiphoton microscopy (MMM) has recently become an important tool in biomedicine for performing three-dimensional fast fluorescence imaging. Using various beamsplitting techniques, MMM splits the near-infrared laser beam into multiple beamlets and produces a multifocal array on the sample for parallel multiphoton excitation and then records fluorescence signal from all foci simultaneously with an area array detector, which significantly improves the imaging speed of multiphoton microscopy and allows for high efficiency in use of the excitation light. In this paper, we discuss the features of several MMM setups using different beamsplitting devices, including a Nipkow spinning disk, a microlens array, a set of beamsplitting mirrors, or a diffractive optical element (DOE). In particular, we present our recent work on the development of an MMM using a spatial light modulator (SLM).

中文翻译：

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多焦点多光子显微镜的最新进展

多焦点多光子显微镜 (MMM) 最近已成为生物医学中用于执行三维快速荧光成像的重要工具。MMM采用多种分束技术，将近红外激光束分成多个子束，在样品上产生多焦点阵列，用于平行多光子激发，然后用面阵探测器同时记录所有焦点的荧光信号，显着提高了样品的成像速度。多光子显微镜，并允许高效率地使用激发光。在本文中，我们讨论了使用不同分束设备的几种 MMM 设置的特征，包括 Nipkow 旋转圆盘、微透镜阵列、一组分束镜或衍射光学元件 (DOE)。特别是，