The biospeckle phenomenon is used for non-destructive monitoring the quality of fresh fruits and vegetables, but in the case of plant tissues there is a lack of experimentally confirmed information about the biological origin of the biospeckle activity (BA). As a main sources of BA, processes associated with the movement inside the cell, such as cytoplasmic streaming, organelle movement and intra- and extracellular transport mechanisms, are considered. The aim of this study is to investigate the effect of metabolism inhibitors, connected with intracellular movement such as cytochalasin B, lantrunculin B, colchicine, cycloheximid, dimethyl sulfoxide (DMSO) and mixture of ion channel inhibitors, injected into apples, on BA. Two methods of BA analysis based on cross-correlation coefficient and Laser Speckle Contrast Analysis (LASCA) were used. DMSO, lantrunculin B and mixture of ion channel inhibitors have a significant effect on BA, and approximately 74 % of BA of apple tissue is potentially caused by biological processes. Results indicate that the functioning of actin microfilaments and ion channels significantly affect BA.

中文翻译：

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Cytochalasin B、Lantrunculin B、秋水仙碱、放线菌酮、二甲基亚砜和离子通道抑制剂对苹果组织中生物斑点活性的影响。

生物斑点现象用于无损监测新鲜水果和蔬菜的质量，但在植物组织的情况下，缺乏关于生物斑点活性 (BA) 生物起源的实验证实信息。作为 BA 的主要来源，考虑了与细胞内运动相关的过程，例如细胞质流、细胞器运动和细胞内外运输机制。本研究的目的是研究与细胞内运动相关的代谢抑制剂，如细胞松弛素 B、lantrunculin B、秋水仙碱、放线菌酮、二甲基亚砜 (DMSO) 和离子通道抑制剂混合物，注射到苹果中对 BA 的影响。使用了基于互相关系数和激光散斑对比度分析 (LASCA) 的两种 BA 分析方法。二甲基亚砜，lantrunculin B 和离子通道抑制剂的混合物对 BA 有显着影响，大约 74% 的苹果组织 BA 可能是由生物过程引起的。结果表明肌动蛋白微丝和离子通道的功能显着影响 BA。