Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.

中文翻译：

---

通过塑造 900 到 1160 nm 的光纤超连续谱进行多模态非线性显微镜

非线性显微镜已广泛用于生物光子成像。脉冲整形控制超快脉冲的非线性光学过程，用于选择性成像和对比度增强。在这项研究中，非线性显微镜，包括双光子荧光、二次谐波产生和三次谐波产生，是使用从 900 到 1160 nm 的光纤超连续谱 (SC) 形成的脉冲进行的。通过将来自 Yb:KYW 脉冲激光器的脉冲耦合到光子晶体光纤中产生的 SC 使用空间光调制器进行光谱过滤和压缩。整形脉冲用于细胞和组织样本的非线性光学成像。光纤 SC 的幅度和相位整形通过单光束和易于调节的设置在宽带宽上提供选择性和高效的非线性光学成像。