In the fast expanding field of combinatorial chemistry, profiling libraries has always been a matter of concern--as illustrated by the buoyant literature over the past seven years. Spectroscopic methods, including especially mass spectrometry and to a lesser extent IR and NMR, have been applied at different levels of combinatorial library synthesis: in the rehearsal phase to optimize the chemistry prior to library generation, to confirm library composition, and to characterize after screening each structure that exhibits positive response. Most of the efforts have been concentrated on library composition assessment. The difficulties of such analyses have evolved from the infancy of the combinatorial concept, where large mixtures were prepared, to the recent parallel syntheses of collections of discrete compounds. Whereas the complexity of the analyses has diminished, an increased degree of automation was simultaneously required to achieve efficient library component identification and quantification. In this respect, mass spectrometry has been found to be the method of choice, providing rapid, sensitive, and informative analyses, especially when coupled to chromatographic separation. Fully automated workstations able to cope with several hundreds of compounds per day have been designed. After a brief introduction to describe the combinatorial approach, library characterization will be discussed in detail, considering first the solution-based methodologies and secondly the support-bound material analyses.

中文翻译：

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组合化学中的质谱。

在组合化学快速发展的领域中，性能分析库一直是一个令人关注的问题，正如过去七年蓬勃发展的文献所证明的那样。光谱方法，特别是质谱分析，以及在较小程度上的IR和NMR，已在组合文库合成的不同水平上应用：在排练阶段，以优化文库生成之前的化学反应，确认文库组成并在筛选后表征每个显示出积极响应的结构。大多数工作都集中在图书馆组成评估上。此类分析的困难已从最初制备大型混合物的组合概念演变为最近的离散化合物集合的并行合成。尽管分析的复杂性降低了，但同时需要提高自动化程度才能实现有效的文库组分鉴定和定量。在这方面，已发现质谱法是一种选择方法，可提供快速，灵敏和信息丰富的分析，尤其是与色谱分离结合使用时。设计了能够每天处理数百种化合物的全自动工作站。在简要介绍了组合方法之后，将详细讨论库表征，首先考虑基于解决方案的方法，其次考虑与支持相关的材料分析。在这方面，已发现质谱法是一种选择方法，可提供快速，灵敏和信息丰富的分析，尤其是与色谱分离结合使用时。设计了能够每天处理数百种化合物的全自动工作站。在简要介绍了组合方法之后，将详细讨论库表征，首先考虑基于解决方案的方法，其次考虑与支持相关的材料分析。在这方面，已发现质谱法是一种选择方法，可提供快速，灵敏和信息丰富的分析，尤其是与色谱分离结合使用时。设计了能够每天处理数百种化合物的全自动工作站。在简要介绍了组合方法之后，将详细讨论库表征，首先考虑基于解决方案的方法，其次考虑与支持相关的材料分析。