We are interested in investigating whether cancer therapy may alter the mitochondrial redox state in cancer cells to inhibit their growth and survival. The redox state can be imaged by the redox scanner that collects the fluorescence signals from both the oxidized-flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tissues and has been previously employed to study tumor aggressiveness and treatment responses. Here, with the redox scanner we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice were treated with CHOP therapy, i.e., cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) with CHO administration on day 1 and prednisone administration on days 1–5. The Fp content of the treated group was significantly decreased (p = 0.033) on day 5, and the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.048). The decrease of the Fp heterogeneity (measured by the mean standard deviation) had a border-line statistical significance (p = 0.071). The result suggests that the mitochondrial metabolism of lymphoma cells was slightly suppressed and the lymphomas became less aggressive after the CHOP therapy.

中文翻译：

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非霍奇金淋巴瘤异种移植物中的斩波疗法诱导的线粒体氧化还原状态改变

我们有兴趣研究癌症治疗是否可以改变癌细胞中的线粒体氧化还原状态以抑制它们的生长和存活。氧化还原状态可以通过氧化还原扫描仪进行成像，该扫描仪收集来自速冻组织中氧化黄素蛋白 (Fp) 和还原形式的烟酰胺腺嘌呤二核苷酸 (NADH) 的荧光信号，之前已用于研究肿瘤侵袭性和治疗反应。在这里，我们使用氧化还原扫描仪研究了化疗对人类弥漫性大 B 细胞淋巴瘤细胞系 (DLCL2) 的小鼠异种移植物的影响。小鼠接受CHOP治疗，即环磷酰胺（C）+羟基多柔比星（H）+ Oncovin（O）+泼尼松（P），第1天给予CHO，第1-5天给予泼尼松。治疗组的Fp含量在第5天显着降低（p = 0.033），治疗组的线粒体氧化还原状态略低于对照组（p = 0.048）。Fp 异质性的降低（通过平均标准偏差测量）具有临界统计显着性（p = 0.071）。结果表明，淋巴瘤细胞的线粒体代谢受到轻微抑制，并且在 CHOP 治疗后淋巴瘤的侵袭性降低。