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Altered levels of pro-inflammatory cytokines in sickle cell disease patients during vaso-occlusive crises and the steady state condition.
European Cytokine Network ( IF 2.8 ) Pub Date : 2013-04-24 , DOI: 10.1684/ecn.2013.0328
Bijan Keikhaei 1 , Ali Reza Mohseni , Reza Norouzirad , Mastaneh Alinejadi , Somayeh Ghanbari , Fariba Shiravi , Ghasem Solgi
Affiliation  

OBJECTIVE This study aimed to evaluate serum levels of pro-inflammatory cytokines and TGF-β in sickle cell disease (SCD) patients, and to compare the results during vaso-occlusive crisis (VOC) or steady state (StSt) conditions. METHODS 54 SCD patients (37HbSS and 17Sβ(+)Thal) were enrolled in the study and evaluated in two groups as follows; group A consisted of 39 VOC patients and group B comprised 15 StSt patients. Nineteen healthy volunteers were included as controls. Circulating levels of IL-1, IL-6, IL-8, IL-17,TNF-α and TGF-β were measured using ELISA. RESULTS Patients in VOC showed higher mean levels of all cytokines than those found in steady-state patients, but this was only marginally significant for IL-8 levels (P = 0.08). Increased levels of TGF-β and IL-17 were found in StSt patients versus normal controls (P = 0.004 and P<0.0001 respectively). A positive correlation was observed between IL-8 and IL-17 in both groups of patients (P = 0.002 and P = 0.005 respectively). Decreased levels of TNF-α, IL-1β and IL-17 were found in hydroxyurea-treated patients. Additionally, significantly higher levels of IL-6 and IL-8 were observed in hydroxyurea-treated and untreated patients than in controls respectively (P = 0.04 and P = 0.01). CONCLUSIONS Our findings indicate that pro-inflammatory cytokines, especially IL-8 and IL-17, could be used as related markers for assessing disease severity, and consequently therapeutic intervention.

中文翻译:

在血管闭塞性危机和稳定状态期间,镰状细胞病患者的促炎细胞因子水平改变。

目的本研究旨在评估镰状细胞病(SCD)患者的促炎细胞因子和TGF-β的血清水平,并比较血管闭塞性危机(VOC)或稳态(StSt)条件下的结果。方法对54例SCD患者(37HbSS和17Sβ(+)Thal)进行了研究,并分为两组进行了评估。A组由39名VOC患者组成,B组由15名StSt患者组成。包括19名健康志愿者作为对照。使用ELISA测量IL-1,IL-6,IL-8,IL-17,TNF-α和TGF-β的循环水平。结果VOC患者的所有细胞因子平均水平均高于稳定状态患者,但对于IL-8水平而言仅微不足道(P = 0.08)。与正常对照组相比,StSt患者的TGF-β和IL-17水平升高(P = 0.004和P <0)。分别为0001)。两组患者的IL-8和IL-17呈正相关(分别为P = 0.002和P = 0.005)。在羟基脲治疗的患者中发现TNF-α,IL-1β和IL-17的水平降低。另外,在羟基脲治疗的患者和未治疗的患者中观察到的IL-6和IL-8水平明显高于对照组(P = 0.04和P = 0.01)。结论我们的发现表明促炎性细胞因子,尤其是IL-8和IL-17,可用作评估疾病严重程度并因此进行治疗干预的相关标志物。在羟基脲治疗的和未治疗的患者中,分别观察到IL-6和IL-8的水平明显高于对照组(P = 0.04和P = 0.01)。结论我们的发现表明促炎性细胞因子,尤其是IL-8和IL-17,可用作评估疾病严重程度并因此进行治疗干预的相关标志物。在羟基脲治疗的和未治疗的患者中,分别观察到IL-6和IL-8的水平显着高于对照组(P = 0.04和P = 0.01)。结论我们的发现表明促炎性细胞因子,尤其是IL-8和IL-17,可用作评估疾病严重程度并因此进行治疗干预的相关标志物。
更新日期:2019-11-01
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