Mucorales are regarded as the aetiological agents of Mucormycosis. Their capabilities to produce mycotoxins are not profoundly investigated, in contrast to those of the fungi from the generaPenicillium, Aspergillus, orFusarium. The aim of this study was to isolate and identify fungi of the order Mucorales and investigate mycotoxins production. Twelve samples of visibly moulded grass silage and eight samples of damaged whole crop maize silage were analysed. Malt extract agar plates were used for sub cultivation. Three fungal species of the order Mucorales were isolated from grass silage, which were identified by their macro-and micro-morphology asAbsidia corymbifera, Mucor circinelloides andRhizopus stolonifer. The cytotoxicity ofMucor circinelloides extract was analysed using the cytotoxicity test (MTT assay) and the result, showed a low cytotoxicity. Additionally extracts fromAbsidia corymbifera, Mucor circinelloides andRhizopus stolonifer were tested for mycotoxin-production using an LC/MS/MS-based multimycotoxin method. 3-nitropropionic acid was detected in the culture extract ofMucor circinelloides.

中文翻译：

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在圆环毛霉中检测 3-硝基丙酸和细胞毒性。

毛霉菌被认为是毛霉菌病的病原体。与来自青霉属、曲霉属或镰刀菌属的真菌相比，它们产生真菌毒素的能力没有被深入研究。本研究的目的是分离和鉴定毛霉目真菌并调查霉菌毒素的产生。分析了 12 个明显霉变的草青贮样品和 8 个受损的整株玉米青贮样品。麦芽提取物琼脂平板用于继代培养。从青贮青贮饲料中分离出三种毛霉目真菌，通过宏观和微观形态学将其鉴定为Absidia corymbifera、Mucor circinelloides和Rhizopus stolonifer。使用细胞毒性试验（MTT测定）分析圆环毛霉提取物的细胞毒性，结果，显示出低细胞毒性。此外，还使用基于 LC/MS/MS 的多真菌毒素方法测试了来自 Absidia corymbifera、Mucor circinelloides 和 Rhizopus stolonifer 的真菌毒素产生量。在圆环毛霉培养物中检测到3-硝基丙酸。