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Investigation of the distribution of deoxynivalenol and ochratoxin A contamination within a 26 t truckload of wheat kernels.
Mycotoxin Research ( IF 3 ) Pub Date : 2008-06-01 , DOI: 10.1007/bf02985287
S Biselli 1 , C Persin , M Syben
Affiliation  

A study was conducted to determine the distribution of deoxynivalenol (DON) and ochratoxin A (OTA) in a lot of 261 of wheat kernels. Within this study, two different sampling and sample preparation strategies were carried out. On the one hand, following the official commission regulation 401/2006/EC, an aggregate sample out of 100 incremental samples was build, homogenized and prepared for laboratory analysis. On the other hand each individual subsample was investigated for its deoxynivalenol and ochratoxin A content. The determined concentration of DON in the individual samples was in a range from 830 up to 2655 (μg/kg, for OTA results ranged from < 0.2 up to 8.6 μg/kg. Thus, a coefficient of variance of 25% for DON and 200% for OTA was achieved. From this, a spot formation for OTA was observed and the average value of the 100 incremental samples did not correspond to the achieved value for the aggregate sample. While the DON contamination at this concentration range seems to be more even, consequently the result of the aggregate sample was in accordance with the average value.In addition a sample communition study was performed to answer the question whether the time consuming process of grinding of the whole aggregate sample is necessary or not. The results of this study show that contamination of whole wheat kernels with DON is at the same level within a 1 kg sample (CV 16%), while OTA contamination shows high variability (CV 94%). At least for OTA this study indicated that an extensive and complete sample communition of the high volume aggregate sample is necessary.

中文翻译:

对 26 吨卡车小麦籽粒中脱氧雪腐镰刀菌烯醇和赭曲霉毒素 A 污染的分布情况进行调查。

进行了一项研究,以确定 261 粒小麦籽粒中脱氧雪腐镰刀菌烯醇 (DON) 和赭曲霉毒素 A (OTA) 的分布。在这项研究中,进行了两种不同的采样和样品制备策略。一方面,根据官方委员会法规 401/2006/EC,从 100 个增量样品中收集总样品,并进行均质化并准备用于实验室分析。另一方面,研究了每个单独的子样品的脱氧雪腐镰刀菌烯醇和赭曲霉毒素 A 的含量。单个样品中 DON 的测定浓度范围为 830 至 2655(μg/kg,OTA 结果范围为 < 0.2 至 8.6 μg/kg。因此,DON 和 200 的变异系数为 25% % 为 OTA 实现。由此,观察到 OTA 的斑点形成,并且 100 个增量样本的平均值与聚合样本的实现值不符。而该浓度范围内的 DON 污染似乎更均匀,因此聚集样品的结果与平均值一致。是否需要整个汇总样本。该研究的结果表明,1 公斤样品中的全麦粒被 DON 污染处于相同水平(CV 16%),而 OTA 污染显示出高度可变性(CV 94%)。至少对于 OTA 而言,这项研究表明,大量聚合样本的广泛而完整的样本交流是必要的。
更新日期:2019-11-01
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