Asparagus spears collected from a total of six commercial plantings in Austria during the main harvest periods in May and June of 2003 and 2004 were examined for endophytic colonization byFusarium spp., particularlyF. proliferatum. Potentially toxigenic fungi such asF. proliferatum were isolated and identified by morphological characteristics using light microscopy. Fumonisin B1 inF. proliferatum-infected asparagus spears was detected with IAS-HPLC-FLD or HPLC-MS/MS. The identity of endophytic fungi colonizing of a total of 816 individual spears was determined. The incidence of infection byF. proliferatum and otherFusarium spp. was highly dependent on location and sampling date. The dominantFusarium species among the endophytic microflora wasF. oxysporum. Other frequently isolated species includedF. proliferatum, F. sambucinum, F. culmorum, F. avenaceum andF. equiseti. The incidence ofF. proliferatum-infected asparagus spears was less than 10% at four of the six sampling locations. At the two remaining locations, 20-47% of the spears examined were infected withF. proliferatum. Further exploration of FB1 generation in asparagus is required because the low levels of FB1 (10-50 (μg/kg) detected in harvested spears in 2003 and 2004 cannot be explained by the results of this study.

中文翻译：

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[Spargelstangenuntersuchungen zur Haupterntezeit auf Infektionen mitFusarium spp。und Kontaminationen mit Fumonisin B1 主要收获期间芦笋矛镰刀菌感染和伏马菌素 B1 污染的调查]。

在 2003 年 5 月和 6 月和 2004 年的主要收获期期间，从奥地利总共六个商业种植园收集的芦笋矛被镰刀菌属（Fusarium spp.）特别是 F. 增殖。潜在的产毒真菌，如 F. 使用光学显微镜通过形态特征分离和鉴定 proliferatum。伏马菌素 B1 inF。用 IAS-HPLC-FLD 或 HPLC-MS/MS 检测被增殖物感染的芦笋。确定了总共 816 个单独矛的内生真菌定植的身份。F.感染的发生率 proliferatum 和其他镰刀菌属。高度依赖于位置和采样日期。内生菌群中的优势镰刀菌种是F. 尖孢菌。其他经常孤立的物种包括 F. proliferatum, F. sambucinum, F. culmorum, F. 白藜芦醇和 F. 木贼。F的发生率 在六个采样位置中的四个位置，被增殖物感染的芦笋长矛少于 10%。在剩下的两个位置，20-47% 的被检查的矛被 F 感染。增殖。需要进一步探索芦笋中 FB1 的产生，因为本研究的结果无法解释 2003 年和 2004 年收获的长矛中检测到的低水平 FB1（10-50 (μg/kg)）。