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Advances in multiplex PCR: balancing primer efficiencies and improving detection success.
Methods in Ecology and Evolution ( IF 6.6 ) Pub Date : 2012-06-28 , DOI: 10.1111/j.2041-210x.2012.00215.x
Daniela Sint 1 , Lorna Raso , Michael Traugott
Affiliation  

1. Multiplex PCR is a valuable tool in many biological studies but it is a multifaceted procedure that has to be planned and optimised thoroughly to achieve robust and meaningful results. In particular, primer concentrations have to be adjusted to assure an even amplification of all targeted DNA fragments. Until now, total DNA extracts were used for balancing primer efficiencies; however, the applicability for comparisons between taxa or different multiple‐copy genes was limited owing to the unknown number of template molecules present per total DNA.

中文翻译:

多重 PCR 的进展:平衡引物效率和提高检测成功率。

1.多重 PCR 在许多生物学研究中是一种有价值的工具,但它是一个多方面的过程,必须彻底规划和优化才能获得可靠和有意义的结果。特别是,必须调整引物浓度以确保所有目标 DNA 片段的均匀扩增。到目前为止,总 DNA 提取物用于平衡引物效率。然而,由于每个总 DNA 中存在的模板分子数量未知,因此在分类群或不同多拷贝基因之间进行比较的适用性受到限制。
更新日期:2012-06-28
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