Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biological applications and represents an interesting model system for the screening applications due to its uniform shape, the advantageous gas supply, and the small volume. However, its application has so far been limited to non#x02010;adherent and aggregate forming cells. Here, we describe for the first time the proof‐of‐principle regarding the adherent cultivation of human embryonic stem cells in HD. For this microcarriers were added to the droplet as dynamic cultivation surfaces resulting in a maintained pluripotency and proliferation capacity for 10 days. This enables the HD technique to be extended to the cultivation of adherence‐dependent stem cells. Also, the possible automation of this method by implementation of liquid handling systems opens new possibilities for miniaturized screenings, the improvement of cultivation and differentiation conditions, and toxicity and drug development.

中文翻译：

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在悬滴系统中成功进行基于表面的人类胚胎干细胞培养的第一步

细胞培养程序的小型化和并行化是研究的重点，以开发具有低材料消耗和更高的毒性和药物筛选标准化的测试平台。悬滴 (HDs) 培养是一种方便且通用的生物应用工具，由于其形状均匀、有利的气体供应和小体积，代表了一种有趣的筛选应用模型系统。然而，迄今为止，其应用仅限于非#x02010；粘附和聚集形成细胞。在这里，我们首次描述了关于 HD 中人胚胎干细胞贴壁培养的原理验证。为此，将微载体作为动态培养表面添加到液滴中，从而在 10 天内保持多能性和增殖能力。这使得 HD 技术能够扩展到粘附依赖性干细胞的培养。此外，通过实施液体处理系统，该方法可能实现的自动化为小型化筛选、培养和分化条件的改善以及毒性和药物开发开辟了新的可能性。