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A new generation of food-borne pathogen detection based on ribosomal RNA.
Annual Review of Food Science and Technology ( IF 12.4 ) Pub Date : 2013-03-08 , DOI: 10.1146/annurev-food-050412-104448
Kristin Livezey 1 , Shannon Kaplan , Michele Wisniewski , Michael M Becker
Affiliation  

Listeria and Salmonella detection assays for food and environmental surfaces that target ribosomal RNA (rRNA) have been developed. The large number of rRNA molecules in bacteria enabled the development of molecular assays that use enrichment times as short as 12 hours for Salmonella and 24 hours for Listeria. These assays run on a fully automated molecular pathogen detection system, which provides walk-away capability and produces 300 assay results in eight hours.

中文翻译:

基于核糖体RNA的新一代食源性病原体检测。

已经开发出针对核糖体RNA(rRNA)的食品和环境表面的李斯特菌和沙门氏菌检测方法。细菌中大量的rRNA分子使分子分析的发展成为可能,沙门氏菌的富集时间短至12小时,李斯特菌的富集时间短至24小时。这些测定法在全自动分子病原体检测系统上运行,该系统具有自动检测功能,并在8小时内产生300个测定结果。
更新日期:2013-03-06
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