Tumor necrosis factor-alpha (TNF-α) could be considered as potential biomarkers in atopic dermatitis (AD), while its level could be influenced by cytokine single gene polymorphisms (SNP). This study was performed in 89 pediatric patients with AD and 137 controls to assess polymorphisms of the TNF-α gene at positions -308 and -238, using the polymerase chain reaction and the sequence-specific primers method. The highest positive allelic association that made the patients susceptible to AD was seen for TNF-α -238/G (p<0.001) and TNF-α -308/G (p = 0.003). The GG genotypes at TNF-α -238 and TNF-α -308, were both significantly higher in the patients with AD, compared to the controls (p<0.01). The GG haplotype at TNF-α (-308,-238) was seen in 92.7% of the patients, which was significantly higher than the controls (p<0.001), while a negative haplotypic association with AD was seen for TNF-α (-308, -238) AG and GA (p<0.01). This study showed that the AG genotype of TNF-α -308, associated with a high production of cytokines, was significantly decreased in patients with AD, while the low-producing GG genotype, which could lead to low production of TNF-α, was over-expressed in the atopic patients.

中文翻译：

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特应性皮炎中的TNF-α单核苷酸多态性。

肿瘤坏死因子-α（TNF-α）被认为是特应性皮炎（AD）的潜在生物标志物，而其水平可能受到细胞因子单基因多态性（SNP）的影响。使用聚合酶链反应和序列特异性引物方法对89例患有AD的儿科患者和137名对照进行了研究，以评估TNF-α基因在-308和-238位的多态性。TNF-α-238 / G（p <0.001）和TNF-α-308 / G（p = 0.003）导致患者对AD敏感的最高阳性等位基因关联。与对照组相比，AD患者的TNF-α-238和TNF-α-308的GG基因型均显着较高（p <0.01）。在92.7％的患者中观察到TNF-α的GG单倍型（-308，-238），显着高于对照组（p <0.001），TNF-α（-308，-238）AG和GA的单倍型与AD呈负相关（p <0.01）。这项研究表明，AD患者的AG基因型TNF-α-308与细胞因子的高产量有关，显着降低，而低产量的GG基因型可能导致TNF-α的低产量。在特应性患者中过度表达。