The aim of the present study was to investigate the protective effect of β-carotene on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rat. Forty male rats were randomly divided into sham, control (I/R injury) and three β-carotene-pretreated groups. To induce the I/R lesions, the celiac artery was clamped for 30 min. The clamp was then removed to allow reperfusion for three hours. Pretreated-rats received β-carotene (15, 30 or 60 mg/kg daily, i.p.) or vehicle for five days before the induction of the I/R injury. Samples of gastric mucosa were collected to measure the mRNA expression of IL-1β, TNF-α and TGF-β by quantitative, real-time PCR. Pretreatment with β-carotene decreased the total area of gastric ulcer and mRNA expression, as well as plasma levels of pro-inflammatory cytokines, IL-1β and TNF-α, in a dose-dependent manner. The gene expression and plasma levels of the anti-inflammatory cytokine, TGF-β, were significantly increased in β-carotene-pretreated groups compared with the control. Our findings showed that the protective effect of β-carotene may be mediated partly by reducing mRNA expression and plasma levels of IL-1β and TNF-α, and concurrently, by increasing gene expression and plasma levels of the anti-inflammatory cytokine TGF-β. These findings suggest that β-carotene has a protective role in gastric mucosa. Further clinical and in vivo studies need to be undertaken to support this hypothesis.

中文翻译：

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β-胡萝卜素可保护胃粘膜免受大鼠缺血再灌注损伤。

本研究的目的是研究β-胡萝卜素对大鼠缺血再灌注（I / R）损伤引起的胃粘膜损伤的保护作用。将40只雄性大鼠随机分为假手术，对照组（I / R损伤）和三个β-胡萝卜素预处理组。为了诱发I / R病变，将腹腔动脉夹住30分钟。然后取下夹子，允许再灌注三小时。预处理大鼠在诱发I / R损伤之前接受β-胡萝卜素（每天15、30或60 mg / kg，腹腔注射）或媒介物，持续5天。收集胃粘膜样品以通过定量实时PCR测量IL-1β，TNF-α和TGF-β的mRNA表达。β-胡萝卜素预处理可减少胃溃疡的总面积和mRNA表达，以及促炎细胞因子IL-1β和TNF-α的血浆水平，以剂量依赖的方式。与对照组相比，β-胡萝卜素预处理组的抗炎细胞因子TGF-β的基因表达和血浆水平显着增加。我们的发现表明，β-胡萝卜素的保护作用可能部分地通过降低IL-1β和TNF-α的mRNA表达和血浆水平，以及同时通过增加抗炎性细胞因子TGF-β的基因表达和血浆水平来介导。 。这些发现表明β-胡萝卜素在胃粘膜中具有保护作用。需要进行进一步的临床和体内研究以支持该假设。我们的发现表明，β-胡萝卜素的保护作用可能部分地通过降低IL-1β和TNF-α的mRNA表达和血浆水平，以及同时通过增加抗炎性细胞因子TGF-β的基因表达和血浆水平来介导。 。这些发现表明β-胡萝卜素在胃粘膜中具有保护作用。需要进行进一步的临床和体内研究以支持该假设。我们的发现表明，β-胡萝卜素的保护作用可能部分地通过降低IL-1β和TNF-α的mRNA表达和血浆水平，以及同时通过增加抗炎性细胞因子TGF-β的基因表达和血浆水平来介导。 。这些发现表明β-胡萝卜素在胃粘膜中具有保护作用。需要进行进一步的临床和体内研究以支持该假设。