This study aims to assess the effects of Ag particles synthesised by a chemical (Ag 20, 200 nm) and biological method (Ag 23, 27 nm) in aquatic organisms: the bacterium Vibrio fischeri, the alga Desmodesmus subspicatus and the crustacean Daphnia magna. Ag particles exerted toxic effects in all organisms studied with Ag particles 23 nm being the most potent. Although soluble Ag was released in all media, the differences between the tested Ag particles still cannot be explained solely based on soluble Ag. NanoSIMS analysis performed with D. magna showed that apart from their localisation in the gut lumen, Ag 200 nm and Ag NPs 23 nm seemed to pass through the epithelial barrier as well. Ag NPs 23 nm localised in specific areas seemed to be within the ovaries. This study strengthens the argument that size, method of synthesis as well as surface chemistry may affect the uptake and toxic effects of Ag NPs.

中文翻译：

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Ag纳米颗粒：对模型水生生物的大小和表面依赖性以及通过NanoSIMS进行吸收评估。

这项研究旨在评估通过化学方法（Ag 20，200 nm）和生物学方法（Ag 23，27 nm）合成的Ag颗粒对水生生物的影响：费氏弧菌，藻类Desmodesmus subspicatus和甲壳类水蚤。在研究的所有生物中，Ag颗粒都具有毒性作用，其中23 nm的Ag颗粒最有效。尽管可溶性Ag在所有介质中均被释放，但仅基于可溶性Ag仍无法解释被测Ag颗粒之间的差异。用D. magna进行的NanoSIMS分析表明，除了它们在肠腔中的定位之外，Ag 200 nm和23 nm的Ag NPs似乎也都穿过上皮屏障。Ag NPs 23 nm位于特定区域似乎在卵巢内。这项研究强化了有关大小，