The effects of different temperature-acclimations on cellular proliferation and migration were studied in the cerebral cortex of the tropical lizard, Tropidurus hispidus. Lizards were divided in two groups: warm-acclimated lizards (WALs), maintained at the temperature and photoperiod conditions of their natural habitat (mean temperature 26°C; 12:12 light:dark) and the cold-acclimated lizards (CALs), maintained at the same cycle of illumination and a mean temperature of 16°C. Animals were injected with the proliferative marker 5-bromodeoxyuridine (BrdU) and euthanized fifteen or thirty days later for the immunostaining. There was no difference in the number of BrdU-positive nuclei between the experimental groups in any of the cortical layers. In CALs, the positive nuclei were found mostly close to the ependyma, whereas in WALs many positive nuclei were also found in the plexiform and cellular layers of the cortex. In CALs, BrdU-positive nuclei appeared grouped (of 2-3 nuclei), a characteristic not seen in the other group. These data suggest that temperature affects the migrating capability of the newly generated neurons in the lizard cortex, but appears not to interfere with its generation.

中文翻译：

---

低温适应会损害热带蜥蜴Tropidurus hispidus（Spix，1825）（Squamata：Tropiduridae）的成年大脑皮层中的细胞迁移。

研究了热带蜥蜴Tropidurus hispidus大脑皮层中不同温度适应对细胞增殖和迁移的影响。蜥蜴分为两组：温驯的蜥蜴（WAL），保持在其自然栖息地的温度和光周期条件下（平均温度26°C； 12：12光照：黑暗）和冷驯化的蜥蜴（CAL），保持在相同的照明周期和16°C的平均温度下。给动物注射增生标记物5-溴脱氧尿苷（BrdU），并在十五或三十天后实施安乐死以进行免疫染色。在任何皮层中，实验组之间的BrdU阳性核数没有差异。在CAL中，发现阳性核大多靠近室管膜，而在WAL中，在皮质的丛状和细胞层中也发现了许多阳性核。在CAL中，BrdU阳性细胞核出现了（2-3个细胞核），这是另一组中没有的特征。这些数据表明温度会影响蜥蜴皮层中新生成的神经元的迁移能力，但似乎不会干扰其生成。