Treatment-induced apoptosis of cancer cells is one goal of cancer therapy. Interestingly, more heat is generated by mitochondria during apoptosis, especially the uncoupled apoptotic state,1,2compared to the resting state. In this case study, we explore these thermal effects by longitudinally measuring temperature variations in a breast lesion of a pathological complete responder during neoadjuvant chemotherapy (NAC). Diffuse Optical Spectroscopic Imaging (DOSI) was employed to derive absolute deep tissue temperature using subtle spectral features of the water peak at 975 nm.3A significant temperature increase was observed in time windows during the anthracycline and cyclophosphamide (AC) regimen but not in the paclitaxel and bevacizumab regimen. Hemoglobin concentration changes generally did not follow temperature, suggesting the measured temperature increases were likely due to mitochondrial uncoupling rather than a direct vascular effect. A simultaneous increase of tissue oxygen saturation with temperature was observed, suggesting that oxidative stress also contributes to apoptosis. Although preliminary, this study indicates longitudinal DOSI tissue temperature monitoring provides information that can improve our understanding of the mechanisms of tissue response during NAC.

中文翻译：

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乳腺癌新辅助化疗期间细胞凋亡引起的深层组织温度变化的非侵入性测量：案例研究

治疗诱导的癌细胞凋亡是癌症治疗的目标之一。有趣的是，线粒体在细胞凋亡过程中会产生更多的热量，尤其是非耦合的凋亡状态，1,2与静止状态相比。在本案例研究中，我们通过纵向测量新辅助化疗 (NAC) 期间病理完全反应者乳房病变的温度变化来探索这些热效应。漫反射光谱成像 (DOSI) 用于使用 975 nm 处水峰的细微光谱特征得出绝对深层组织温度。3在蒽环类和环磷酰胺 (AC) 方案的时间窗中观察到显着的温度升高，但在紫杉醇和贝伐单抗方案中没有。血红蛋白浓度的变化通常不随温度变化，这表明测得的温度升高可能是由于线粒体解偶联而不是直接的血管效应。观察到组织氧饱和度随温度同时增加，这表明氧化应激也有助于细胞凋亡。虽然是初步的，但这项研究表明，纵向 DOSI 组织温度监测提供的信息可以提高我们对 NAC 期间组织反应机制的理解。