Human platelets are thought to express approximately 2000-3000 proteins, but post-translational modifications, alternatively spliced variants and a rich diversity of vertebrate domain architectures likely make this a conservative estimate. Even though rapidly advancing proteomic techniques have facilitated the identification of roughly one third of the platelet proteome, a combination of abundance-based and activity-based proteomics methodologies is needed for elucidation of platelet functional characteristics including the definition of a "core proteome" and recognition of diverse enzyme activity profiles associated with various physiological states. In this review, we describe the latest mass spectrometry-based techniques capable of providing some of these physiological details required for more comprehensive evaluation of the human platelet repertoire.

中文翻译：

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血小板生物学中基于丰度和活性的蛋白质组学

人类血小板被认为表达大约 2000-3000 种蛋白质，但翻译后修饰、选择性剪接变体和丰富多样的脊椎动物域结构可能使这一估计成为保守的估计。尽管快速发展的蛋白质组学技术促进了大约三分之一的血小板蛋白质组的鉴定，但仍需要结合基于丰度和基于活性的蛋白质组学方法来阐明血小板功能特征，包括“核心蛋白质组”的定义和识别与各种生理状态相关的不同酶活性谱。在这次审查中，