Data on genetic polymorphisms associated with response to anti-HIV drugs has accumulated over the years. Information on how polymorphisms influence drug metabolism and transport to target sites is important in guiding dosage or selection of appropriate alternative therapies. This study determined the frequency of MDR1 C3435T and CYP2B6 G516T polymorphisms associated with the transport and metabolism of efavirenz and nevirapine, in a population of South African HIV infected patients. In addition, association of polymorphisms with immunologic and virologic factors was investigated. A 207bp of MDR1 exon 26 and a 161bp of CYP2B6 exon 4 were obtained from patients by polymerase chain reaction. Analysis of population-based sequences of MDR1 revealed a frequency of 89% and 11% of C and T alleles respectively (n=197; X² = 0.974; p=0.324). Restriction fragment length polymorphism (RFLP) analysis of the CYP2B6 gene revealed a prevalence of 9.5% of GG, 78.4% of GT and 12.1% of TT genotype (n= 199; X² = 65.204; p=0.00). There was no significant difference between immune recovery and decline in viral load (n=53), with genotype after repeated calculations of analysis of variance (ANOVA).

中文翻译：

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MDR1 C3435T 和 CYP2B6 G516T 多态性在 HIV-1 感染的南非患者中的流行。

多年来，与抗 HIV 药物反应相关的遗传多态性数据已经积累。关于多态性如何影响药物代谢和转运到靶位点的信息对于指导剂量或选择合适的替代疗法很重要。本研究确定了与依非韦伦和奈韦拉平的转运和代谢相关的 MDR1 C3435T 和 CYP2B6 G516T 多态性在南非 HIV 感染患者群体中的频率。此外，研究了多态性与免疫学和病毒学因素的关联。通过聚合酶链反应从患者体内获得207bp的MDR1外显子26和161bp的CYP2B6外显子4。基于群体的 MDR1 序列分析显示 C 和 T 等位基因的频率分别为 89% 和 11%（n=197; X ² = 0.974；p = 0.324）。CYP2B6 基因的限制性片段长度多态性 (RFLP) 分析揭示了 9.5% 的 GG、78.4% 的 GT 和 12.1% 的 TT 基因型（n = 199；X ² = 65.204；p = 0.00）。免疫恢复和病毒载量下降之间没有显着差异（n = 53），重复计算方差分析（ANOVA）后的基因型。