With the development of next-generation sequencing technology, ever-expanding databases of genetic information from various organisms are available to researchers. However, our ability to study the biological meaning of genetic information and to apply our genetic knowledge to produce genetically modified crops and animals is limited, largely due to the lack of molecular tools to manipulate genomes. Recently, targeted cleavage of the genome using engineered DNA scissors called zinc finger nucleases (ZFNs) has successfully supported the precise manipulation of genetic information in various cells, animals, and plants. In this review, we will discuss the development and applications of ZFN technology for genome engineering and highlight recent reports on its use in plants.

中文翻译：

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通过锌指核酸酶进行靶向基因组工程。

随着新一代测序技术的发展，越来越多的来自各种生物的遗传信息数据库可供研究人员使用。然而，我们研究遗传信息的生物学意义和应用我们的遗传知识生产转基因作物和动物的能力是有限的，这主要是由于缺乏操纵基因组的分子工具。最近，使用称为锌指核酸酶 (ZFN) 的工程 DNA 剪刀对基因组进行靶向切割已成功支持对各种细胞、动物和植物中遗传信息的精确操作。在这篇综述中，我们将讨论 ZFN 技术在基因组工程中的发展和应用，并重点介绍最近有关其在植物中使用的报告。