The template-directed oligomerization of nucleoside-5'-phosphoro-2-methyl imidazolides on standard oligonucleotide templates has been studied extensively. Here, we describe experiments with templates in which inosinic acid (I) is substituted for guanylic acid, or 2,6-diaminopurine nucleotide (D) for adenylic acid. We find that the substitution of I for G in a template is strongly inhibitory and prevents any incorporation of C into internal positions in the oligomeric products of the reaction. The substitution of D for A, on the contrary, leads to increased incorporation of U into the products. We found no evidence for the template-directed facilitation of oligomerization of A or I through A-I base pairing. The significance of these results for prebiotic chemistry is discussed.

中文翻译：

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在包含肌苷酸或二氨基嘌呤核苷酸残基的模板上进行非酶促低聚反应。

已经广泛研究了标准寡核苷酸模板上核苷5'-磷酸-2-甲基咪唑啉化物的模板指导寡聚。在这里，我们描述了使用模板进行的实验，其中肌苷酸（I）替代了鸟苷酸，或2,6-二氨基嘌呤核苷酸（D）替代了腺苷酸。我们发现，在模板中用G取代I是很强的抑制作用，并且可以防止C掺入反应低聚产物的内部位置。相反，用D代替A会导致U掺入产品的数量增加。我们没有发现通过AI碱基配对通过模板指导的A或I寡聚化的证据。讨论了这些结果对益生元化学的意义。