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Effect of microgravity and hypergravity on embryo axis alignment during postencystment embryogenesis in Artemia franciscana (Anostraca).
Journal of Crustacean Biology ( IF 1.1 ) Pub Date : 1995-11-01
J R Rosowski 1 , M A Gouthro , K K Schmidt , B J Klement , B S Spooner
Affiliation  

Cysts of brine shrimp attached with a liquid adhesive to 12-mm diameter glass coverslips in a syringe-type fluid processing apparatus were flown aboard the NASA space shuttle Discovery, flight STS-60, from 3-11 February 1994, and were allowed to undergo postencystment embryogenesis and to hatch in microgravity. The shuttle flight and the ground-based control coverslips with attached cysts were parallel to the earth's surface during incubation in salt water. Based on the position of the cyst shell crack in the attached cyst population, the ground-control nauplii emerged mostly upward. On the shuttle in microgravity, although our method of detection of orientation would not reveal emergence toward the coverslip, the ratio of the position of the cyst shell crack in the population after hatching best fit the predicted values of a random direction for nauplii emergence. Centrifugation on earth was then used to create hypergravity forces of up to 73 g during postencystment embryogenesis and hatching. The upward orientation of emerging nauplii showed a high degree of correlation (r(2) =98.8%) with a linear relationship to the log of g, with 78.2% of the total hatching upward at 1 g and 91.0% hatching upward at 73 g.

中文翻译:

Franciscana(Anostraca)的胚胎发育后微重力和超重力对胚胎轴排列的影响。

1994年2月3日至11日,在注射器式流体处理设备中,将液态虾附着在直径12毫米的玻璃盖玻片上的盐水虾囊肿乘上NASA探索号STS-60航天飞机,并允许其进行胚胎后发生并在微重力中孵化。在盐水中孵育期间,航天飞机飞行和附有囊肿的地面控制盖玻片与地球表面平行。根据囊肿在附生囊肿中的位置,地面控制无节幼体大部分向上出现。在微重力的航天飞机上,尽管我们的定向检测方法不会显示出盖玻片的出现,孵化后人群中囊壳裂缝位置的比例最适合无节幼体出现的随机方向的预测值。然后在地球上离心后在胚胎发生和孵化过程中产生高达73 g的超重力。出现的无节幼体的向上方向显示出与g的对数呈线性关系的高度相关性(r(2)= 98.8%),在1 g时向上孵化的总比例为78.2%,在73 g时向上孵化的比例为91.0% 。
更新日期:2019-11-01
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