Intracellular labile iron is an iron species which is not or weakly bound to proteins and depicts an important effect on homeostatic regulation in cells. An excess or deficiency of iron can cause oxidative damage to key cellular biomolecules. The behavior and concentrations of labile iron are difficult to monitor, but the specific redox state of the Fe ions is relevant to the physiological and pathological properties that we would like to study. We have developed a series of turn-on type fluorescent probes that are highly selective to the labile Fe(II) ions, and we have tested their applications to cellular level imaging. These probes are based on N-oxide chemistry with a range of fluorophores that depict optimal performance for specific applications. Herein, I review the recent progress of our research and discuss prospects for future work to understand the relation between intracellular ion and oxidative stress.

中文翻译：

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用于活细胞中Fe（II）离子成像的化学工具的开发：综述。

细胞内不稳定的铁是一种不与蛋白质结合或与蛋白质结合弱的铁，对细胞体内的稳态调节具有重要作用。铁的过量或缺乏会导致关键细胞生物分子的氧化损伤。不稳定铁的行为和浓度很难监测，但是Fe离子的特定氧化还原状态与我们要研究的生理和病理特性有关。我们已经开发了一系列对不稳定的Fe（II）离子具有高度选择性的开启型荧光探针，并且已经测试了它们在细胞水平成像中的应用。这些探针基于N-氧化物化学，带有一系列荧光团，描绘了特定应用的最佳性能。在此处，