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Circulating natural antibodies to inflammatory cytokines are potential biomarkers for atherosclerosis.
Journal of Inflammation ( IF 5.1 ) Pub Date : 2018-11-16 , DOI: 10.1186/s12950-018-0199-2
Peng Wang 1 , Huan Zhao 1 , Zhenqi Wang 2 , Xuan Zhang 1
Affiliation  

Background Inflammatory cytokines contribute to the development of atherosclerosis. Natural antibodies in the circulation have protective effects on common diseases including atherosclerosis-related conditions. Purpose The present study aimed to investigate the possible involvement of circulating IgG antibodies against inflammatory cytokines in atherosclerosis. Methods A total of 220 patients with diagnosis of atherosclerosis and 200 healthy controls were recruited. Seven linear peptide antigens were used to develop an enzyme-linked immunosorbent assay in-house for detection of plasma IgG antibodies against interleukin 1β (fragments IL1β-1 and IL1β-2), IL6, IL8, tumor necrosis factor alpha (fragments TNFα-1 and TNFα-2) and IL1α. Results Atherosclerotic patients had an increase in the levels of circulating IgG to TNFα-1(adjusted r2 = 0.038, p < 0.001) and IL1α (adjusted r2 = 0.025, p = 0.002) compared with control subjects. Female patients mainly contributed to increased anti-TNFα-1 IgG levels (adjusted r2 = 0.073, p < 0.001) and anti-IL1α IgG levels (adjusted r2 = 0.044, p = 0.003). In addition, female patients showed higher anti-IL1β-2 IgG levels than controls (adjusted r2 = 0.023, p = 0.026). There was no significant change of circulating IgG antibodies to other cytokines. ROC curve analysis showed an AUC of 0.564 for anti-TNFα-1 IgG assay with 22.8% sensitivity against a specificity of 90.0%, and an AUC of 0.539 for anti-IL1α IgG assay with 17.8% sensitivity against a specificity of 90.0%; the anti-IL1β-2 IgG assay had an AUC of 0.580 with 26.3% sensitivity against a specificity of 89.8% in female patients. There was no correlation between plasma IgG levels and carotid intima-media thickness. Conclusion Natural antibodies to inflammatory cytokines may be potential biomarkers for atherosclerosis.

中文翻译:

循环中针对炎性细胞因子的天然抗体是动脉粥样硬化的潜在生物标志物。

背景炎性细胞因子有助于动脉粥样硬化的发展。循环中的天然抗体对包括动脉粥样硬化相关疾病在内的常见疾病具有保护作用。目的 本研究旨在探讨循环中抗炎性细胞因子的 IgG 抗体可能参与动脉粥样硬化。方法共招募220名确诊为动脉粥样硬化的患者和200名健康对照者。七种线性肽抗原用于内部开发酶联免疫吸附试验,用于检测针对白细胞介素 1β(IL1β-1 和 IL1β-2 片段)、IL6、IL8、肿瘤坏死因子 α(TNFα-1 片段)的血浆 IgG 抗体和 TNFα-2) 和 IL1α。结果 动脉粥样硬化患者的循环 IgG 对 TNFα-1 水平升高(调整后的 r2 = 0.038,与对照组相比,p < 0.001)和 IL1α(调整后的 r2 = 0.025,p = 0.002)。女性患者主要导致抗 TNFα-1 IgG 水平升高(调整后的 r2 = 0.073,p < 0.001)和抗 IL1α IgG 水平(调整后的 r2 = 0.044,p = 0.003)。此外,女性患者的抗 IL1β-2 IgG 水平高于对照组(调整后的 r2 = 0.023,p = 0.026)。其他细胞因子的循环 IgG 抗体没有显着变化。ROC 曲线分析显示,抗 TNFα-1 IgG 测定的 AUC 为 0.564,敏感性为 22.8%,特异性为 90.0%;抗 IL1α IgG 测定的 AUC 为 0.539,敏感性为 17.8%,特异性为 90.0%;抗 IL1β-2 IgG 检测的 AUC 为 0.580,敏感性为 26.3%,而女性患者的特异性为 89.8%。血浆 IgG 水平与颈动脉内膜中层厚度之间没有相关性。结论 炎性细胞因子的天然抗体可能是动脉粥样硬化的潜在生物标志物。
更新日期:2020-04-22
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