ABSTRACT Magnetic hyperthermia is a fast emerging, non-invasive cancer treatment method which is used synergistically with the existing cancer therapeutics. We have attempted to address the current challenges in clinical magnetic hyperthermia-improved biocompatibility and enhanced heating characteristics, through a single combinatorial approach. Both superparamagnetic iron oxide nanoparticles (SPIONs) of size 10 nm and ferrimagnetic iron oxide nanoparticles (FIONs) of size 30 nm were synthesized by thermal decomposition method for comparison studies. Two different surface modifying agents, viz, Cetyl Trimethyl Ammonium Bromide and 3-Aminopropyltrimethoxysilane, were used to conjugate Bovine Serum Albumin (BSA) over the iron oxide nanoparticles via two different methods-surface charge adsorption and covalent amide bonding, respectively. The preliminary haemolysis and cell viability experiments show that BSA conjugation mitigates the haemolytic effect of the iron oxide nanoparticles on erythrocytes and is non-cytotoxic to the healthy Baby Hamster Kidney cells. It was observed from the results that due to better colloidal stability, the SAR value of the BSA-iron oxide nanoparticles is higher than the iron oxide nanoparticles without BSA, irrespective of the size of the iron oxide nanoparticles and method of conjugation. The BSA-FIONs seem to show improved biocompatibility, as the haemolytic index is less than 2 % and cell viability is up to 120 %, when normalized with the control. The SAR value of BSA-FIONs is 2300 W g-1 when compared to 1700 W g-1 of FIONs without BSA conjugation. Thus, we report here that BSA conjugation over FIONs (with a high saturation magnetization of 87 emu g-1) provide a single combinatorial approach to improve the biocompatibility and enhance the SAR value for magnetic hyperthermia, thus addressing both the current challenges of the same. GRAPHICAL ABSTRACT

中文翻译：

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牛血清白蛋白共轭亚铁磁性氧化铁纳米颗粒增强生物相容性和磁热疗性能。

摘要：磁热疗是一种快速兴起的非侵入性癌症治疗方法，可与现有的癌症疗法协同使用。我们试图通过单一的组合方法来解决当前临床磁热疗改善生物相容性和增强加热特性方面的挑战。通过热分解法合成了尺寸为 10 nm 的超顺磁性氧化铁纳米粒子 (SPIONs) 和尺寸为 30 nm 的亚铁磁性氧化铁纳米粒子 (FIONs) 用于比较研究。两种不同的表面改性剂，即十六烷基三甲基溴化铵和 3-氨基丙基三甲氧基硅烷，分别通过两种不同的方法——表面电荷吸附和共价酰胺键合——将牛血清白蛋白 (BSA) 缀合在氧化铁纳米颗粒上。初步的溶血和细胞活力实验表明，BSA 结合可减轻氧化铁纳米颗粒对红细胞的溶血作用，并且对健康的小仓鼠肾细胞无细胞毒性。从结果中观察到，由于更好的胶体稳定性，BSA-氧化铁纳米颗粒的SAR值高于没有BSA的氧化铁纳米颗粒，而与氧化铁纳米颗粒的尺寸和缀合方法无关。BSA-FION 似乎显示出改进的生物相容性，因为溶血指数低于 2%，细胞活力高达 120%，当与对照标准化时。BSA-FION 的 SAR 值为 2300 W g-1，而未结合 BSA 的 FION 的 SAR 值为 1700 W g-1。因此，我们在此报告 FIONs 上的 BSA 共轭（具有 87 emu g-1 的高饱和磁化强度）提供了一种单一的组合方法来提高生物相容性并提高磁热疗的 SAR 值，从而解决当前面临的两个挑战。图形概要