The spread of infectious diseases and the increase in the drug resistance among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of the ecofriendly procedure for the synthesis of nanoparticles is growing day-by-day in the field of nanobiotechnology. In the present study, we use the extract of green alga Botryococcus braunii for the synthesis of copper and silver nanoparticles. The characterization of copper and silver nanoparticles was carried out by using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron spectroscopy (SEM). FTIR measurements showed all functional groups having control over reduction and stabilization of the nanoparticles. The X-ray diffraction pattern revealed that the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay.

中文翻译：

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绿藻布鲁尼球菌生物合成铜和银纳米粒子及其抗菌活性。

微生物之间传染病的传播和耐药性的增加迫使研究人员合成具有生物活性的纳米粒子。在纳米生物技术领域中，纳米颗粒合成的生态友好程序的改进正在日趋发展。在本研究中，我们使用了绿藻Bratryi的提取物用于合成铜和银纳米粒子。铜和银纳米粒子的表征是通过使用UV-Vis光谱，傅里叶变换红外光谱（FTIR），X射线衍射（XRD）和扫描电子光谱（SEM）进行的。FTIR测量显示所有官能团均具有控制纳米颗粒还原和稳定的功能。X射线衍射图表明，该颗粒本质上是晶体，具有面心立方（FCC）的几何形状。SEM显微照片显示了生物合成的金属纳米颗粒的形态。此外，发现这些生物合成的纳米颗粒对两种革兰氏阴性细菌铜绿假单胞菌（MTCC 441）和大肠杆菌均具有高毒性。（MTCC 442），两种革兰氏阳性细菌肺炎克雷伯菌（MTCC 109）和金黄色葡萄球菌（MTCC 96），以及真菌菌株尖孢镰刀菌（MTCC 2087）。通过琼脂孔板法测量抑制区域，此外，通过肉汤稀释测定法确定最小抑制浓度。