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Efficacy of collagen and alginate hydrogels for the prevention of rat chondrocyte dedifferentiation.
Journal of Tissue Engineering ( IF 8.2 ) Pub Date : 2018-10-08 , DOI: 10.1177/2041731418802438
Guang-Zhen Jin 1, 2 , Hae-Won Kim 1, 2, 3
Affiliation  

Dedifferentiation of chondrocytes remains a major problem in cartilage tissue engineering. The development of hydrogels that can preserve chondrogenic phenotype and prevent chondrocyte dedifferentiation is a meaningful strategy to solve dedifferentiation problem of chondrocytes. In the present study, three gels were prepared (alginate gel (Alg gel), type I collagen gel (Col gel), and their combination gel (Alg/Col gel)), and the in vitro efficacy of chondrocytes culture while preserving their phenotypes was investigated. While Col gel became substantially contracted with time, the cells encapsulated in Alg gel preserved the shape over the culture period of 14 days. The mechanical and cell-associated contraction behaviors of Alg/Col gel were similar to those of Alg. The cells in Alg and Alg/Col gels exhibited round morphology, whereas those in Col gel became elongated (i.e. fibroblast-like) during cultures. The cells proliferated with time in all gels with the highest proliferation being attained in Col gel. The expression of chondrogenic genes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated in Alg/Col gel and Col gel, particularly in Col gel. However, the chondrocyte dedifferentiation markers, type I collagen and alkaline phosphatase (ALP), were also expressed at significant levels in Col gel, which being contrasted with the events in Alg and Alg/Col gels. The current results suggest the cells cultured in hydrogels can express chondrocyte dedifferentiation markers as well as chondrocyte markers, which draws attention to choose proper hydrogels for chondrocyte-based cartilage tissue engineering.

中文翻译:

胶原蛋白和藻酸盐水凝胶预防大鼠软骨细胞去分化的功效。

软骨细胞的去分化仍然是软骨组织工程中的一个主要问题。开发能够保留软骨形成表型并防止软骨细胞去分化的水凝胶是解决软骨细胞去分化问题的一种有意义的策略。本研究制备了三种凝胶(海藻酸盐凝胶(Alg gel)、I型胶原凝胶(Col gel)及其组合凝胶(Alg/Col gel)),并在保留软骨细胞表型的同时进行体外培养软骨细胞的功效被调查。虽然 Col 凝胶随着时间的推移而大幅收缩,但封装在 Alg 凝胶中的细胞在 14 天的培养期内保持了形状。Alg/Col 凝胶的机械和细胞相关收缩行为与 Alg 相似。Alg 和 Alg/Col 凝胶中的细胞呈现圆形形态,而 Col 凝胶中的细胞在培养过程中变得拉长(即成纤维细胞样)。所有凝胶中的细胞都随着时间的推移而增殖,其中在 Col 凝胶中增殖最高。软骨形成基因(包括 SOX9、II 型胶原蛋白和聚集蛋白聚糖)的表达在 Alg/Col 凝胶和 Col 凝胶中显着上调,特别是在 Col 凝胶中。然而,软骨细胞去分化标记物、I 型胶原和碱性磷酸酶 (ALP) 在 Col 凝胶中也以显着水平表达,这与 Alg 和 Alg/Col 凝胶中的事件形成对比。目前的结果表明,在水凝胶中培养的细胞可以表达软骨细胞去分化标记物以及软骨细胞标记物,这引起了人们对选择合适的水凝胶用于基于软骨细胞的软骨组织工程的关注。
更新日期:2019-11-01
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