BackgroundThis study aimed to validate the function of CKX gene on grain numbers in wheat.Methodswe constructed and transformed a RNA interference expression vector of TaCKX2.4 in bread wheat line NB1. Southern blotting analysis was used to select transgenic plants with single copy. The expression of TaCKX2.4 gene was estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, the relation between expression of TaCKX2.4 gene and grain numbers was validated.ResultsTotally, 20 positive independent events were obtained. Homozygous lines from 5 events with a single copy of transformed gene each were selected to evaluate the expression of TaCKX2.4 and grain numbers per spike in T3 generation. Compared with the control NB1, the average grain numbers per spike significantly increased by 12.6%, 8.3%, 6.5% and 5.8% in the T3 lines JW39-3A, JW1-2B, JW1-1A and JW5-1A, respectively.ConclusionOur study indicated that the expression level of TaCKX2.4 was negatively correlated with the grain number per spike, indicating that the reduced expression of TaCKX2.4 increased grain numbers per spike in wheat.

中文翻译：

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小麦细胞分裂素氧化酶2基因RNA干扰提高每穗粒数

背景本研究旨在验证CKX基因对小麦粒数的影响。方法构建并转化了面包小麦NB1系中TaCKX2.4的RNA干扰表达载体。Southern印迹分析用于选择具有单拷贝的转基因植物。TaCKX2.4 基因的表达通过定量实时PCR (qRT-PCR) 分析估计。最后验证了TaCKX2.4基因表达与籽粒数的关系。结果共获得20个阳性独立事件。选择来自 5 个事件的纯合系，每个事件具有一个单拷贝的转化基因，以评估 TaCKX2.4 的表达和 T3 代中每个穗的粒数。与对照NB1相比，T3系JW39-3A、JW1-2B、