ABSTRACT Ammonia and Nitrate Bio-removal Over Nitrite (ANBON) is a new biological process, which couples denitratation with Anaerobic Ammonium Oxidation (ANAMMOX) to carry out simultaneous removal of nitrate nitrogen and ammonia nitrogen. The effect of hydraulic retention time (HRT) on the performance of ANBON process was investigated. The results showed that the optimal HRT was about 0.7 h and the nitrogen removal rate was 26.2 ± 0.7 g N·L−1·d−1, which was at top level reported in previous literatures. The change of HRT was found to trigger the change of microbial community in the reactor, which exerted a great effect on the performance of ANBON process. The community analysis based on 16S rRNA gene indicated that Halomonas and Candidatus Kuenenia were the dominant functional bacteria in the denitratation unit and the ANAMMOX unit respectively. These results are helpful for the development and application of ANBON process. GRAPHICAL ABSTRACT

中文翻译：

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水力停留时间对亚硝酸盐过程中氨和硝酸盐生物去除的影响

摘要 亚硝酸氨和硝酸盐生物去除（ANBON）是一种新的生物工艺，将反硝化与厌氧氨氧化（ANAMMOX）相结合，同时去除硝态氮和氨氮。研究了水力停留时间 (HRT) 对 ANBON 工艺性能的影响。结果表明，最佳HRT约为0.7 h，脱氮率为26.2±0.7 g N·L-1·d-1，处于以往文献报道的最高水平。发现HRT的变化会引发反应器中微生物群落的变化，这对ANBON工艺的性能产生了很大的影响。基于16S rRNA基因的群落分析表明，Halomonas和Candidatus Kuenenia分别是脱硝单元和厌氧氨氧化单元的优势功能菌。这些结果有助于ANBON工艺的开发和应用。图形概要