当前位置:
X-MOL 学术
›
Environ. Sci. Eur.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Systematic miRNome profiling reveals differential microRNAs in transgenic maize metabolism.
Environmental Sciences Europe ( IF 5.9 ) Pub Date : 2018-09-19 , DOI: 10.1186/s12302-018-0168-7 Sarah Zanon Agapito-Tenfen 1 , Vinicius Vilperte 2, 3 , Terje Ingemar Traavik 1 , Rubens Onofre Nodari 2
中文翻译:
系统性 miRNA 分析揭示了转基因玉米代谢中的差异 microRNA。
更新日期:2018-09-19
Environmental Sciences Europe ( IF 5.9 ) Pub Date : 2018-09-19 , DOI: 10.1186/s12302-018-0168-7 Sarah Zanon Agapito-Tenfen 1 , Vinicius Vilperte 2, 3 , Terje Ingemar Traavik 1 , Rubens Onofre Nodari 2
Affiliation
Background
While some genetically modified organisms (GMOs) are created to produce new double-stranded RNA molecules (dsRNA), in others, such molecules may occur as an unintended effect of the genetic engineering process. Furthermore, GMOs might produce naturally occurring dsRNA molecules in higher or lower quantities than its non-transgenic counterpart. This study is the first to use high-throughput technology to characterize the miRNome of commercialized GM maize events and to investigate potential alterations in miRNA regulatory networks.Results
Thirteen different conserved miRNAs were found to be dys-regulated in GM samples. The insecticide Bt GM variety had the most distinct miRNome. These miRNAs target a range of endogenous transcripts, such as transcription factors and nucleic acid binding domains, which play key molecular functions in basic genetic regulation. In addition, we have identified 20 potential novel miRNAs with target transcripts involved in lipid metabolism in maize. isomiRs were also found in 96 conserved miRNAs sequences, as well as potential transgenic miRNA sequences, which both can be a source of potential off-target effects in the plant genome. We have also provided information on technical limitations and when to carry on additional in vivo experimental testing.Conclusions
These findings do not reveal hazards per se but show that robust and reproducible miRNA profiling technique can strengthen the assessment of risk by detecting any new intended and unintended dsRNA molecules, regardless of the outcome, at any stage of GMO development.中文翻译:
系统性 miRNA 分析揭示了转基因玉米代谢中的差异 microRNA。