The continual renewal of the epidermis is thought to be related to the presence of populations of epidermal stem cells residing in physically protected microenvironments (rete ridges) directly influenced by the presence of mesenchymal fibroblasts. Current skin in vitro models do acknowledge the influence of stromal fibroblasts in skin reorganisation but the study of the effect of the rete ridge-microenvironment on epidermal renewal still remains a rich topic for exploration. We suggest there is a need for the development of new in vitro models in which to study epithelial stem cell behaviour prior to translating these models into the design of new cell-free biomaterial devices for skin reconstruction. In this study, we aimed to develop new prototype epidermal-like layers containing pseudo-rete ridge structures for studying the effect of topographical cues on epithelial cell behaviour. The models were designed using a range of three-dimensional electrospun microfabricated scaffolds. This was achieved via the utilisation of polyethylene glycol diacrylate to produce a reusable template over which poly(3-hydrroxybutyrate-co-3-hydroxyvalerate) was electrospun. Initial investigations studied the behaviour of keratinocytes cultured on models using plain scaffolds (without the presence of intricate topography) versus keratinocytes cultured on scaffolds containing microfeatures.

中文翻译：

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一种生产微加工电纺膜的方法，用于创建新的皮肤再生模型。

表皮的持续更新被认为与存在于受物理保护的微环境（网脊）中的表皮干细胞群的存在有关，这些微环境直接受到间充质成纤维细胞的存在的影响。目前的皮肤体外模型确实承认基质成纤维细胞在皮肤重组中的影响，但视网膜脊微环境对表皮更新的影响的研究仍然是一个有待探索的丰富课题。我们建议需要开发新的体外模型来研究上皮干细胞行为，然后将这些模型转化为用于皮肤重建的新型无细胞生物材料装置的设计。在这项研究中，我们的目的是开发包含伪网状脊结构的新原型表皮样层，以研究地形线索对上皮细胞行为的影响。该模型是使用一系列三维静电纺丝微加工支架设计的。这是通过利用聚乙二醇二丙烯酸酯生产可重复使用的模板来实现的，在该模板上进行电纺丝聚（3-羟基丁酸酯-co-3-羟基戊酸酯）。初步研究研究了使用普通支架（不存在复杂地形）的模型上培养的角质形成细胞与包含微观特征的支架上培养的角质形成细胞的行为。