Abstract

The possibility of adapting the Standard Addition Method (SAM) to calibration in very difficult analytical conditions, namely when there is a need to determine an analyte with the use of nonlinear calibration graph and in the presence of matrix components causing additive interference effect, is investigated. To this aim the SAM in the common version and the Chemical H-point Standard Addition Method (C-HPSAM) realized by the flow injection technique were applied. Specifically, a flow manifold was used for construction of a set of nonlinear calibration graphs in different chemical conditions. As the graphs were intersected indicating both the additive interference effect and the analytical result free of this effect, the analyte concentration in the sample was able to be obtained with improved accuracy. The applicability of this approach was verified on the example of spectrophotometric determination of paracetamol in pharmaceuticals and of total acidity in wines. The C-HPSAM method enabled complete compensation of the additive effect and obtaining analytical results at a relative error not exceeding 6.0%.

Graphical abstract

中文翻译：

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在存在加性干扰效应的情况下，通过非线性标准添加方法进行可靠校准。

摘要

研究了在非常困难的分析条件下调整标准添加法 (SAM) 以进行校准的可能性，即当需要使用非线性校准图确定分析物并且存在导致加性干扰效应的基质成分时. 为此，应用了普通版本的 SAM 和通过流动注射技术实现的化学 H 点标准添加法 (C-HPSAM)。具体而言，流歧管用于构建不同化学条件下的一组非线性校准图。由于图形相交表明了加性干扰效应和没有这种效应的分析结果，因此能够以更高的准确度获得样品中的分析物浓度。以分光光度法测定药物中的扑热息痛和葡萄酒中的总酸度为例，验证了该方法的适用性。C-HPSAM 方法能够完全补偿加性效应并以不超过 6.0% 的相对误差获得分析结果。

图形概要